Showing: 161 - 180 of 180 results
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Plasmid#159099PurposeCckA FRET Sensor with DITE motif mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationCckA D293 mutated to A293 and V199I on mRuby3 (P…PromoterXyloseAvailabilityAcademic Institutions and Nonprofits only -
pGWKS7
Plasmid#139414PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
pPEE46
Plasmid#139425PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B2
Plasmid#172843PurposeCRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mRuby3
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV_ACTB CRISPIE
Plasmid#172853PurposeActb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.DepositorInsertsActb intron 1 sgRNA
DRS-2 sgRNA
CRISPIE designer exon (phase 0-0) encoding mEGFP
mRuby3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGD431
Plasmid#132382Purposecontains the sequence of mRuby3for C-term fusions in the pGGD000 backbone (GreenGate)DepositorInsertmRuby3
UseGreengate entry plasmidTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSWD55
Plasmid#158618PurposeShort Fibronectin FRET standardDepositorInsertShort FRET Standard
UseTagsExpressionBacterialMutationPromoterXyloseAvailabilityAcademic Institutions and Nonprofits only -
pSWD56
Plasmid#158619PurposeLong Fibronectin FRET StandardDepositorInsertLong FRET Standard
UseTagsExpressionBacterialMutationPromoterXyloseAvailabilityAcademic Institutions and Nonprofits only -
pKL10-mRuby-iATPSnFR1.0 bb118
Plasmid#158980PurposeConstitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.DepositorInsertmruby-iATPSNfR1.0
UseTagsmruby3ExpressionBacterialMutationcodon optimized for e. coli from Addgene#102557PromoterBba_J23118 (sigma 70)AvailabilityAcademic Institutions and Nonprofits only -
pSWD95
Plasmid#158617PurposeCckA FRET SensorDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationPromoterXyloseAvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-cyto-Ruby3-iATPSnFR1.0
Plasmid#102554Purposeexpresses cytoplasmic ATP sensor in astrocytes with red reference proteinDepositorInsertmRuby3-iATPSnFR1.0
UseAAVTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSWD95Y14D
Plasmid#159098PurposeCckA FRET Sensor with cyclic-di-GMP point mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationMutated Y514 to D514PromoterXyloseAvailabilityAcademic Institutions and Nonprofits only -
pGilesSHdI_psmyc_His6_triple red
Plasmid#170047PurposeMono-Responsive Reporter Gene Constructs for triple redDepositorInsertsmScarlet
COmScarlet
mRuby3
UseUnspecifiedTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSiMPls_N + pSiMPls_C
Plasmid#167203PurposeSiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycinDepositorInsertsEGFP
SmR 1-75 + gp41-1 N-intein
mRuby3
gp41-1 C-intein + SmR 76-263
UseTagsExpressionBacterialMutationPromoterAmpR, CAT, pBAD, and pTrcAvailabilityAcademic Institutions and Nonprofits only -
pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green
Plasmid#170048PurposeTri-Responsive Reporter Gene Construct for redgreen, triple red, and double greenDepositorInsertsmScarlet
COmScarlet
mRuby3
mWasabi dN
mWasabi CO
UseUnspecifiedTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB:Psmyc triple red
Plasmid#169413PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple redDepositorInsertsmScarlet proper
COmScarlet
mRuby3
UseUnspecifiedTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEK39
Plasmid#210599PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
UseTagsmRuby3ExpressionBacterial and YeastMutationPromoterSpH2BproAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-RCC
Plasmid#190635PurposeMammalian expression of genetically encoded calcium indicator RCCDepositorInsertRCC
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FNL Combinatorial Cloning Platform
Plasmid Kit#1000000211PurposeA collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systemsDepositorAvailabilityAcademic Institutions and Nonprofits only -
Diatom uLoop Assembly Kit
Plasmid Kit#1000000238PurposeThe collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.DepositorAvailabilityAcademic Institutions and Nonprofits only
Showing: 161 - 180 of 180 results