Addgene

We narrowed to 640 results for: pBR322

Showing: 161 - 180 of 640 results

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pCMK105

Plasmid

#226805

Purpose

Integrates KanR into glpK, 570 bp homology, three homologous HIP1 sites in left homology arm

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK195

Plasmid

#226818

Purpose

Integrates KanR into NS2, 1250 bp homology, two homologous HIP1 sites in each of the right and left homology arms

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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MTF1

Plasmid

#205066

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Tang et al J Biol Chem. 2009 Feb 27;284(9):5514-22.

Insert

MTF1 (MTF1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBac{9Xmef2-eGFP,attB,w+}

Plasmid

#182197

Purpose

EGFP driven by MEF2 transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBac{9Xtin-eGFP,w+,attB}

Plasmid

#182194

Purpose

EGFP driven by tinman transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBac{3Xtin-GAGA-eGFP,w+,attB}

Plasmid

#182195

Purpose

EGFP driven by tinman and GAGA transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBac{3Xmef2-eGFP,attB,w+}

Plasmid

#182196

Purpose

EGFP driven by MEF2 transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBac{3Xtin-eGFP,w+,attB}

Plasmid

#182193

Purpose

EGFP driven by tinman transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJH572

Plasmid

#172156

Purpose

Negative control pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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spoT-

Plasmid

#100913

Purpose

deletes synpcc7942_1377 (spoT)

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

Kanamycin casette (in place of spoT gene) flanked by S. elongatus sequence

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC

Plasmid

#101828

Purpose

drives expression of kaiBC from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kanamycin casette and p0050 promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::glgP_gap1

Plasmid

#101845

Purpose

Replaces native promoter of glgP_gap1 with Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

CmR casette followed by ptrc promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::zwf_fbp_opcA

Plasmid

#101846

Purpose

Replaces native promoter of zwf_fbp_opcA with Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

sp/st resistance casette followed by ptrc promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA

Plasmid

#101830

Purpose

drives expression of rpaA from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ gentamicin resistance casette)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::gnd

Plasmid

#101844

Purpose

drives expression of gnd using Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kan casette followed by ptrc promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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zwf-fbp-opcA-

Plasmid

#101839

Purpose

deletes zwf_fbp_opcA locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of synpcc7942_2333-2335 operon)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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MSP969

Plasmid

#98802

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-ENLYFHS-TEV T146S, D148P, S153N, S170A, N177M

Use

Tags

Expression

Bacterial

Mutation

T146S, D148P, S153N, S170A, N177M

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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MSP824

Plasmid

#98803

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-ENLYGQS-TEV V209M, W211I, M218F

Use

Tags

Expression

Bacterial

Mutation

V209M, W211I, M218F

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCVD30

Plasmid

#63691

Purpose

pCVD25 with the Tcr gene removed, XbaI is intact, 540 bp of CT A is deleted. Cloned from V. cholerae 395 chromosome.

Depositor

James Kaper

Article

Kaper et al Nature Biotechnology 2, 345 - 349 (1984)

Insert

V. cholera chromosomal DNA

Use

Tags

Expression

Bacterial

Mutation

540 bp of CT A is deleted

Promoter

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
pC0037

Plasmid

#181934

Purpose

Dual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

Gaussia luciferase
Cypridina luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and EF1alpha

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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