We narrowed to 13,096 results for: plasmids 101
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Plasmid#135250PurposeGateway destination clone of TLK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTLK2-V5-TurboID (TLK2 Human)
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-TLK2_IDG-K
Plasmid#135278PurposeGateway destination clone of TLK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-TLK2 (TLK2 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-7aa-cb5-pEGFP-C1
Plasmid#177407PurposeTo express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.DepositorInsertCb5 tail anchor (Cyb5r4 Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-TLK2-V5-miniTurbo_IDG-K
Plasmid#135249PurposeGateway destination clone of TLK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTLK2-V5-miniTurbo (TLK2 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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KIF3B
Plasmid#25254PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKIF3B:S6-Q359 (KIF3B Human)
UseTagsHisExpressionBacterialMutationContains amino acids S6-Q359PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRE-T2-IRES-His6-Ubiquitin
Plasmid#107107PurposeDesigned to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.DepositorInserthuman ubiquitin UBB (UBB Human)
UseTags6xHisExpressionMammalianMutationPromoterTetAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A D491A HA pcDNA3
Plasmid#206109Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin bindingDepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationR241A D491APromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
AASS (AASSA-c013)
Plasmid#98227PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertAASSA (AASS Human)
UseBaculovirusTagsHis-6-TEVExpressionMutationpfam03435:Saccharop_dh 483-916PromoterpolyhedrinAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK4 Ankyrin Repeats pET151
Plasmid#164057PurposeExpresses mouse KANK4 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDZ2FE_Champ1_1-812_2PA
Plasmid#105676PurposeExpresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 bindingDepositorInsertChamp1 (CHAMP1 Human)
UseTags2X Flag tagExpressionMammalianMutationMutations: P336A, P341APromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRESN2_GFP_YTA_Champ1_328-354
Plasmid#105639PurposeExpresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFPDepositorInsertChamp1 (CHAMP1 Human)
UseTagsGFP YTAExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
His6-Halo-TEV-Akt2
Plasmid#87606Purposeexpresses His-halo tagged at the N-terminus of human Akt2 proteinDepositorInsertAKT 2 (AKT2 Human)
UseZebrafish (danio rerio) expressionTagsHis-Halo-TevExpressionMammalianMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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BAT8 (3RJW)
Plasmid#28125PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBAT8 (EHMT2 Human)
UseTagsHis-tag with integrated thrombin siteExpressionBacterialMutationcontains amino acid residues 913-1193.PromoterAvailable sinceFeb. 10, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
HSD17B14
Plasmid#39160PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHSD17B14 (HSD17B14 Human)
UseTagsHis TevExpressionBacterialMutationcodon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
CRISPseq-BFP-backbone
Plasmid#85707PurposeBackbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Pur-CAG-hM3Dq-mCherry
Plasmid#80948Purposehuman AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoterDepositorInserthM3Dq-mCherry
UseTagsmcherry fusionExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Pur-CAG-hM4Di-mCherry
Plasmid#80947Purposehuman AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoterDepositorInserthM4Di-mCherry
UseTagsmcherry fusionExpressionMammalianMutationPromoterCAG promoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only