We narrowed to 29,612 results for: PLE;

1. plenti-px330-CRBN-T1-pGK-Pur

   Plasmid

   #107382

   Purpose

   Mammalian expression CRISPR/Cas9

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   sgRNA targeting CRBN (CRBN Human)
   

   Use

   Lentiviral; Crispr

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD

   Plasmid

   #225703

   Purpose

   Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3 (K36M) (H3-3A, H3-3B Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xAID HA and T2A-mTurquoise

   Expression

   Mammalian

   Mutation

   K36M

   Promoter

   pEF1A

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-EML4-ALK variant 3a I1171N

   Plasmid

   #183832

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Mutation

   I1171N

   Promoter

   CMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CaMKIIa-eArchT 3.0-EYFP

   Plasmid

   #35513

   Purpose

   Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   eArchT 3.0-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Enhanced trafficking signal and ER export signal …

   Promoter

   CaMKIIa

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-FlaviA-mNeptune-puro

   Plasmid

   #140091

   Purpose

   Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

   Insert

   FlaviA-mNeptune
   

   Use

   Lentiviral; High expression

   Tags

   mNeptune

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-EF1a ANXA11-mEmerald D40G

   Plasmid

   #164211

   Purpose

   pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   D40G

   Promoter

   EF1a

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-EF1a-GFP-miRE_shRen-Puro

   Plasmid

   #135683

   Purpose

   Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

   Depositor

   Florian Karreth

   Insert

   shRenilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-GFP-2A-G(N2c)-WPRE

   Plasmid

   #194352

   Purpose

   Lentivirus expressing eGFP and Rabies Glycoprotein (G) from CVS-N2c strain

   Depositor

   Marco Tripodi

   Article

   Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

   Inserts

   Enhanced Green Fluorescent Protein (eGFP)
   Rabies CVS-N2c glycoprotein (G)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX307-SARS-CoV-2-3CL-C145A

   Plasmid

   #160279

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C145A

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV/TO p16 Hygro (w184-1)

    Plasmid

    #22263

    Depositor

    Eric Campeau

    Insert

    cyclin-dependent kinase inhibitor 2A (CDKN2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CMV-GFP-miRE_shSCR-Puro

    Plasmid

    #135689

    Purpose

    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selection

    Depositor

    Florian Karreth

    Insert

    shScrambled
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti X1 Zeo/pTER shMDC1 (673-1)

    Plasmid

    #22193

    Depositor

    Eric Campeau

    Insert

    sh Mediator of DNA damage checkpoint 1
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

    Plasmid

    #20946

    Purpose

    3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    halorhodopsin
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX-FHH-NRASQ61K-IRES-Puro

    Plasmid

    #120570

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-TRE3G-BE3RA-PGK-Puro

    Plasmid

    #110846

    Purpose

    Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    TRE3G

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-tdTomato-miRE_shRen-Puro

    Plasmid

    #135687

    Purpose

    Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-EF1alpha-IRES-EGFP-HA-hNPHP1

    Plasmid

    #122874

    Purpose

    Expresses HA-tagged human NPHP1 in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Insert

    human NPHP1 (NPHP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CaMKIIa-eNpHR 3.0-EYFP

    Plasmid

    #26970

    Purpose

    3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Insert

    eNpHR 3.0
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Trafficking Signal (TS), ER Export Signal (FCYENE)

    Promoter

    CaMKIIa

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti CMV/TO V5-LUC Puro (w549-1)

    Plasmid

    #19785

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX-FHH-NRAS-IRES-Puro

    Plasmid

    #120569

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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