We narrowed to 9,863 results for: 158

1. pOPINK-VCPIP (OTU, aa 25-561)

   Plasmid

   #61583

   Purpose

   Expresses human VCPIP (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   VCPIP (VCPIP1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-24 and aa 562-1222.

   Promoter

   T7

   Available since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-6P-2_dCrk

   Plasmid

   #131146

   Purpose

   Expresses GST::dCrk (based on crk isoform RA).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG

   Plasmid

   #176400

   Purpose

   Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   FRB-RGG-GFP-RGG-RGG
   

   Use

   Tags

   FRB and GFP

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172504

   Purpose

   Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPC-mCh-ACTB-P2A-EGFP

   Plasmid

   #165158

   Purpose

   Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

   Depositor

   Taekjip Ha

   Article

   Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

   Insert

   mCherry-ACTB-P2A-EGFP (ACTB Human)
   

   Use

   Retroviral

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. G1333 DddAtox-C–SaKKH-Cas9(D10A)

   Plasmid

   #157838

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PhyB-mCherry-Spc72

   Plasmid

   #51582

   Purpose

   Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   Spc72 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. G1397 DddAtox-N–SaKKH-Cas9(D10A)

   Plasmid

   #157839

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR221 NLRP1 A66V

   Plasmid

   #166817

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   A66V

   Promoter

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221 NLRP1 S1213A

    Plasmid

    #166824

    Purpose

    Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    S1213A

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQStrep2-GADD45G

    Plasmid

    #31584

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    growth arrest and DNA-damage-inducible, gamma (GADD45G Human)
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgCTRL-hygro

    Plasmid

    #199639

    Purpose

    sgRNA control; induces CAS9 cutting in an intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221 NLRP1 P1214R

    Plasmid

    #166822

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    P1214R

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT2-MEKK3-K392A

    Plasmid

    #21585

    Depositor

    John Kyriakis

    Article

    Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

    Insert

    mitogen-activated protein kinase kinase kinase 3 (MAP3K3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lysine 392 to Arginine

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pscALPS-SIV-AGM-TAN1 vpr

    Plasmid

    #115819

    Purpose

    Encodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVAGM Vpr (TAN1)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPR sgHAL

    Plasmid

    #102315

    Purpose

    genetic depletion of HAL

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgHAL (HAL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MigR1-Flag-TRIB1

    Plasmid

    #131158

    Purpose

    Retroviral expression of flag-tagged mouse TRIB1

    Depositor

    Karen Keeshan, Warren Pear

    Article

    Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

    Insert

    Trib1 (Trib1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221 NLRP1 M77T

    Plasmid

    #166818

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    M77T

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221 NLRP1 A54T

    Plasmid

    #166815

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    A54T

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221 NLRP1 FIIND (F1079M-L1373) S1213A

    Plasmid

    #166832

    Purpose

    Gateway pDONR vector containing NLRP1 FIIND with autoprocessing deficient S1213A mutation (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge