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CBX3 A5.5 gRNA

Plasmid

#90568

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX3 (Guide Designation A5.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-HOXA9

Plasmid

#51302

Purpose

Expression of HOXA9 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

homeobox A9 (HOXA9 Human)

Use

Lentiviral

Tags

n/a

Mutation

n/a

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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RRM1 E2.3 gRNA

Plasmid

#90881

Purpose

3rd generation lentiviral gRNA plasmid targeting human RRM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RRM1 (Guide Designation E2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST15 hSlp4-a

Plasmid

#40046

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a (SYTL4 Human)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
EZH2 E9.3 gRNA

Plasmid

#90684

Purpose

3rd generation lentiviral gRNA plasmid targeting human EZH2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

EZH2 (Guide Designation E9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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RRM2 E4.3 gRNA

Plasmid

#90883

Purpose

3rd generation lentiviral gRNA plasmid targeting human RRM2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RRM2 (Guide Designation E4.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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RRM2 E3.3 gRNA

Plasmid

#90882

Purpose

3rd generation lentiviral gRNA plasmid targeting human RRM2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RRM2 (Guide Designation E3.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 merlin 1-595

Plasmid

#11623

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
EZH2 E10.3 gRNA

Plasmid

#90685

Purpose

3rd generation lentiviral gRNA plasmid targeting human EZH2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

EZH2 (Guide Designation E10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-LZ-CD95L

Plasmid

#104349

Purpose

Expresses human CD95L leucine zipper in mammalian cells

Depositor

Alexander McLellan

Article

Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

Insert

CD95L leucine zipper (FASLG Human)

Tags

octahistidine tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FIH1

Plasmid

#21403

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

HIF1AN (HIF1AN Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
VCP F12.4 gRNA

Plasmid

#90939

Purpose

3rd generation lentiviral gRNA plasmid targeting human VCP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

VCP (Guide Designation F12.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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VCP F11.4 gRNA

Plasmid

#90938

Purpose

3rd generation lentiviral gRNA plasmid targeting human VCP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

VCP (Guide Designation F11.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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CRISPseq-mCherry-backbone

Plasmid

#85708

Purpose

Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.

Depositor

Ido Amit

Article

Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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584 pSG5L HA RB 661W

Plasmid

#10731

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB 661W (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

661W

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
FRAP

Plasmid

#1827

Depositor

Philip Leder

Article

Ornitz et al Mol Cell Biol 1992 Jan;12(1):240-7.

Insert

FR1 AP (Fgfr1 Mouse)

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only

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pLX317-hcRED

Plasmid

#115440

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

hcRED

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-FIH1

Plasmid

#21399

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

HIF1AN (HIF1AN Human)

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pBabe puro H-Ras G12V T35S

Plasmid

#12274

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V T35S (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The T35S mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
MASTL B4.4 gRNA

Plasmid

#90755

Purpose

3rd generation lentiviral gRNA plasmid targeting human MASTL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MASTL (Guide Designation B4.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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