We narrowed to 4,712 results for: lum

1. px330-UFL1 sgRNA1

   Plasmid

   #134635

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA1 (UFL1 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRZ-mNanog-YNL

   Plasmid

   #65717

   Purpose

   mNanog reporter plasmid (expresses YNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   murine Nanog promoter and YNL
   

   Use

   Lentiviral and Luciferase

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. NAX_CAGGS_CAS9_m4_KRAB_KanR neo

   Plasmid

   #167877

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB700 lenti gRNA KanR neo

   Plasmid

   #167909

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-mCherry_SNAP_SNAPV_PACT_Dead

   Plasmid

   #173073

   Purpose

   Dead SNAP tag at the centrosome

   Depositor

   Heidi Hehnly

   Article

   Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

   Insert

   mCherry-SNAP-SNAP-Varied-PACT
   

   Tags

   mCherry-SNAP-SNAP-Varied

   Expression

   Mammalian

   Mutation

   AA144 in both SNAP tags changed from a cysteine t…

   Promoter

   CMV IE94

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDNA3-Cerulean-FHA1-NES

   Plasmid

   #138202

   Purpose

   Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   Cerulean-FHA1-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

   Plasmid

   #192406

   Purpose

   To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::Turbo GFP
   

   Use

   Synthetic Biology

   Tags

   N7

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV Gab1(PRD)-mCherry-HOTag3

   Plasmid

   #190026

   Purpose

   Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

   Depositor

   Lukasz Bugaj

   Article

   Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

   Insert

   Gab1(PRD) CluMPS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. XE241 Rat frizzled-1-GFP-CS2P+

   Plasmid

   #16817

   Depositor

   Randall Moon

   Article

   Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

   Insert

   frizzled-1 (Fzd1 Rat)
   

   Use

   Xenopus expression

   Tags

   GFP

   Available Since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-PKCsub-YPet-NES

    Plasmid

    #138207

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub-YPet-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mGFAP-Halo

    Plasmid

    #169474

    Purpose

    the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    GFAP (Gfap Mouse)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Changed R 236 to H

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. NAX_CAGGS_CAS9_m4_KanR neo

    Plasmid

    #167869

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. NAX_CAGGS_CAS9_m4_VPR_Hygro

    Plasmid

    #167883

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-VHL Y98N-pBabe-puro

    Plasmid

    #19236

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 98 to Asparagin

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYNL-N1_TUBB5

    Plasmid

    #65678

    Purpose

    Expresses beta-tubulin-YNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    beta-tubulin class V
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid

    #185687

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-2xHaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLTR PKC beta II

    Plasmid

    #8417

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Rat Protein Kinase C beta II (Prkcb Rat)
    

    Expression

    Mammalian

    Available Since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

18. HA-VHL Y112N-pBabe-puro

    Plasmid

    #19238

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 112 to Asparagin

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-VHL Y112H-pBabe-puro

    Plasmid

    #19237

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 112 to Histidin

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1a-2xSV40_NLS-CeNLuc

    Plasmid

    #135952

    Purpose

    Expresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    CeNLuc LumiFluor
    

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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