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pDSAY

Plasmid

#62291

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCsn2

Plasmid

#113251

Purpose

E. coli expression vector for S. pyogenes Csn2-MBP-His

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Csn2

Tags

MBP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas1

Plasmid

#113248

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas2

Plasmid

#113249

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas2

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas2

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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GGDestX1-Amp

Plasmid

#157652

Purpose

Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Cell-free expression (e. coli sigma-70)

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDSAG

Plasmid

#62289

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, GFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDSARN

Plasmid

#62295

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDSAT

Plasmid

#62290

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, mTurquoise CFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pNOC-NLUC

Plasmid

#98141

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven NanoLuciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pattB-RfB2

Plasmid

#62296

Purpose

docking site transgenesis plasmid for Gateway Cloning and insect transgenesis

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Unspecified

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDSAYN

Plasmid

#62294

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pRB326

Plasmid

#8667

Depositor

David Botstein

Article

Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Insert

TUB1 (TUB1 Budding Yeast)

Use

Centromeric

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pCAGGS mutated Pdx1 (DM#233)

Plasmid

#15072

Depositor

Douglas Melton

Article

Grapin-Botton et al Genes Dev. 2001 Feb 15. 15(4):444-54.

Insert

Pdx1

Expression

Mammalian

Mutation

DNA binding mutant

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pGLU_119

Plasmid

#225799

Purpose

RS421 library variant 14434 in pGLDV_18 (p15A)

Depositor

Nili Ostrov

Article

Gilbert et al bioRxiv 2025.12.22.696024

Insert

14434_[Tn5]

Use

Synthetic Biology

Mutation

N/A

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGLU_83

Plasmid

#225784

Purpose

RS421 library variant 14434 in IDT backbone

Depositor

Nili Ostrov

Article

Gilbert et al bioRxiv 2025.12.22.696024

Insert

14434_[abr]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSN859

Plasmid

#190692

Purpose

Mammalian expression of kinesin scFv(H2) fused with Fc domain of chicken IgY

Depositor

Shinsuke Niwa

Article

Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

Insert

kinesin scFv(H2)::Fc domain of chicken IgY

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC-FLUC

Plasmid

#98140

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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UB13p::dsSPL10 v01 (SPL10-RNAi)

Plasmid

#27409

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 inverted (AT1G27370 Mustard Weed)

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

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ML40K1

Bacterial Strain

#61920

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA genes. deleted. Contains pACYC plasmid.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Chloramphenicol

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Article

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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