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We narrowed to 4,423 results for: BFP

Showing: 1841 - 1860 of 4423 results

  1. L4219 TNFR2 CTEVp chain in PolyTX-mTagBFP2

    Plasmid
    #244177
    Purpose
    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-CTEVp(190K)-PRS(M)-VP64-ZF6
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1B Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    TNFR2 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244177-plasmid-map-sequence-id-487904

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  2. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-1

    Plasmid
    #200539
    Purpose
    mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites
    Depositor
    Junsu Kang
    Insert
    BFP, NTRv2
    Use
    Mini-golden
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    200539-plasmid-map-sequence-id-394645

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  3. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-2

    Plasmid
    #200540
    Purpose
    mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites
    Depositor
    Junsu Kang
    Insert
    BFP, NTRv2
    Use
    Mini-golden
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    200540-plasmid-map-sequence-id-394646

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  4. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-3

    Plasmid
    #200541
    Purpose
    mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites
    Depositor
    Junsu Kang
    Insert
    BFP, NTRv2
    Use
    Mini-golden
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    200541-plasmid-map-sequence-id-394647

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  5. pMC-ME_2a-BFP-pA ORF-1 without ATG

    Plasmid
    #200534
    Purpose
    mini circle middle entry plasmid for 2a-BFP-pA ORF 1 (without ATG) with flanking BsaI cut sites
    Depositor
    Junsu Kang
    Insert
    BFP
    Use
    Mini-golden
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    200534-plasmid-map-sequence-id-394640

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  6. pMC-ME_2a-BFP-pA ORF-2 without ATG

    Plasmid
    #200535
    Purpose
    mini circle middle entry plasmid for 2a-BFP-pA ORF 2 (without ATG) with flanking BsaI cut sites
    Depositor
    Junsu Kang
    Insert
    BFP
    Use
    Mini-golden
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    200535-plasmid-map-sequence-id-394641

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  7. pMC-ME_2a-BFP-pA ORF-3 without ATG

    Plasmid
    #200536
    Purpose
    mini circle middle entry plasmid for 2a-BFP-pA ORF 3 (without ATG) with flanking BsaI cut sites
    Depositor
    Junsu Kang
    Insert
    BFP
    Use
    Mini-golden
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    200536-plasmid-map-sequence-id-394642

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  11. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

    Plasmid
    #223106
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223106-plasmid-map-sequence-id-447626

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  12. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

    Plasmid
    #223107
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223107-plasmid-map-sequence-id-447623

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  13. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

    Plasmid
    #223108
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223108-plasmid-map-sequence-id-447631

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  14. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

    Plasmid
    #223109
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223109-plasmid-map-sequence-id-447628

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  20. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid
    #163701
    Purpose
    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Inserts
    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    Use
    AAV and CRISPR
    Tags
    3x-HA and P2A-tagBFP
    Expression
    Mammalian
    Promoter
    CAG and TRE3G
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163701-plasmid-map-sequence-id-325288

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Showing: 1841 - 1860 of 4423 results