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We narrowed to 17,882 results for: crispr plasmids

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pMCS-rybozyme-IRES-CAS9

Plasmid

#64668

Purpose

Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

Depositor

Wataru Fujii

Article

Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

Inserts

CAS9
HDV ribozyme

Use

CRISPR

Tags

Flag, Internal ribosome entry site, and guide RNA…

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgRNA

Plasmid

#124844

Purpose

Vector for Cre-dependent expression of SaCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SaCas9, gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS and NLS-3xHA

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

Plasmid

#42335

Purpose

A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pDG459

Plasmid

#100901

Purpose

SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

Depositor

Paul Thomas

Article

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG and T2A-PuroR

Expression

Mammalian

Mutation

Promoter

CBh and U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDG458

Plasmid

#100900

Purpose

SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

Depositor

Paul Thomas

Article

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh and U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET-MBP-NLS-Geo_st

Plasmid

#87703

Purpose

Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

Depositor

Jennifer Doudna

Insert

GeoCas9

Use

Tags

10xHis-MBP-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pNSU299_MET16

Plasmid

#166091

Purpose

Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast

Depositor

Rebecca Burgess

Insert

MET16 (MET16 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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HCP1

Plasmid

#166103

Purpose

This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.

Depositor

Matthias Heinemann

Insert

Msn2-sgRNA#27 (MSN2 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_CPR1_1

Plasmid

#166074

Purpose

Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast

Depositor

Rebecca Burgess

Insert

CPR1 (CPR1 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_RRT8

Plasmid

#166080

Purpose

Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.

Depositor

Rebecca Burgess

Insert

Promoter of RRT8 (RRT8 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_CPR1_2

Plasmid

#166071

Purpose

Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.

Depositor

Rebecca Burgess

Insert

Intergenic region near CPR1

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_GAL7

Plasmid

#166089

Purpose

Plasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeast

Depositor

Rebecca Burgess

Insert

GAL7 (GAL7 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_GLK1

Plasmid

#166090

Purpose

Plasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeast

Depositor

Rebecca Burgess

Insert

GLK1 (GLK1 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_LHS1

Plasmid

#166092

Purpose

Plasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeast

Depositor

Rebecca Burgess

Insert

LHS1 (LHS1 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_RPL22A

Plasmid

#166079

Purpose

Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.

Depositor

Rebecca Burgess

Insert

Promoter of RPL22A (RPL22A Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_YBL055C

Plasmid

#166077

Purpose

Plasmid for constituive spCas9 and tet-inducible YBL055C targeting sgRNA expression for double stranded break formation in yeast

Depositor

Rebecca Burgess

Insert

YBL055C (near PTC3) (YBL055C Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_YNR071C

Plasmid

#166081

Purpose

Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of YNR071C for double stranded break formation in yeast.

Depositor

Rebecca Burgess

Insert

Promoter of YNR071C (YNR071C Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_SLA1

Plasmid

#166075

Purpose

Plasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeast

Depositor

Rebecca Burgess

Insert

SLA1 (SLA1 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_PDR12

Plasmid

#166093

Purpose

Plasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeast

Depositor

Rebecca Burgess

Insert

PDR12 (PDR12 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pNSU299_TDH2

Plasmid

#166082

Purpose

Plasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeast

Depositor

Rebecca Burgess

Insert

TDH2 (TDH2 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Tet-inducible

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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