We narrowed to 19,028 results for: REV

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TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

Plasmid

#89347

Purpose

2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

Jurkat TCRalpha/beta alleles and CD3e and CD3z

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCE35

Plasmid

#174409

Purpose

C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJOG1368

Plasmid

#167301

Purpose

Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

FCY-UPP fusion gene

Use

Synthetic Biology; Moclo level 0 vector

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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dCas12j3 VPR

Plasmid

#188517

Purpose

Expresses FLAG tagged dCas12j3 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTetD-β-tubulin_SNAP

Plasmid

#136832

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-OSH2-2xPH

Plasmid

#161987

Purpose

Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

Depositor

Tamas Balla, David Bryant

Article

Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

Insert

OSH2 (OSH2 Budding Yeast)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAT9658-sgRNA-mCherry

Plasmid

#162987

Purpose

sgRNA cloning plasmid

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

exchangeable cassette

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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AMPK-γ1-HA

Plasmid

#40605

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-γ1 (Prkag1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-PM-ExRai-AktAR2

Plasmid

#184050

Purpose

Plasma membrane-targeted ExRai-AktAR2

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

PM-ExRai-AktAR2

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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p-AAV-sh[SNCA]

Plasmid

#75437

Purpose

knockdown alpha-synuclein in rat neurons

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

snca (Snca Rat)

Use

AAV and RNAi

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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