We narrowed to 15,117 results for: pET

1. csd1_125-312/pET28b(+)

   Plasmid

   #83290

   Purpose

   csd1, construct: 125-312, pET28b(+) NC-tag

   Depositor

   SeWon Suh

   Article

   An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

   Insert

   hp1543
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-124

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet-msGC-NT2

   Plasmid

   #74763

   Purpose

   expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

   Depositor

   William Montfort

   Article

   Hu et al J Biol Chem. 2008 Jul 25;283(30):20968-7

   Inserts

   fragment of alfa subunit of sGC
   fragment of beta subunit of sGC
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   fragment 49-471 of the alfa subunit

   Promoter

   T7

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK044 (pET-PurQ-wt)

   Plasmid

   #73839

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM6-1D4-mCherry

   Plasmid

   #73423

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 1D4 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P1D4 (orthogonal T7-lac variant)

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM6-4A6-mCherry

   Plasmid

   #73424

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 4A6 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P4A6 (orthogonal T7-lac variant)

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-1E4-mCherry

   Plasmid

   #73426

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 1E4 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P1E4 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-1B6-mCherry

   Plasmid

   #73420

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 1B6 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P1B6 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6-3H5-mCherry

   Plasmid

   #73419

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3H5 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P3H5 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET23a-MmUBB(SnaBI)

   Plasmid

   #69558

   Purpose

   In vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBB (Ubb Mouse)
   

   Expression

   Bacterial

   Mutation

   The two last codons (TATTAA; encoding the last ty…

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET23a-MmUBB(Y305P)

    Plasmid

    #69557

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (Ubb Mouse)
    

    Expression

    Bacterial

    Mutation

    Codon TAT encoding tyrosine 305 was changed to CC…

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pET151-hUCH37 (ISF2)

    Plasmid

    #61930

    Purpose

    Expression of hUCH37 (ISF2) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET30Xa-SBL1(160E)

    Plasmid

    #66846

    Purpose

    Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    S160E

    Promoter

    T7lac

    Available Since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11+PCNA3-CYP119

    Plasmid

    #66136

    Purpose

    Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b+PCNA3 T180C

    Plasmid

    #66181

    Purpose

    Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    T180C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b+PCNA3 R112C

    Plasmid

    #66180

    Purpose

    Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15b+PCNA2 E105C

    Plasmid

    #66176

    Purpose

    Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-p53 (Cow 1-82)

    Plasmid

    #62062

    Purpose

    Expression of Cow p53 (residues 1-82) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-82

    Promoter

    T7

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET28-p53 (1-73) (K24N)

    Plasmid

    #62079

    Purpose

    Expression of human p53 (1-73) (K24N) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; K24N

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET28-p53 (Rabbit 1-87)

    Plasmid

    #62058

    Purpose

    Expression of Rabbit p53 (residues 1-87) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-87

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pET28A-p53 (1-73) (PPP)

    Plasmid

    #62067

    Purpose

    Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P12A, P13A, P27A

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pET28-ceRab2(1-214)

    Plasmid

    #59550

    Purpose

    makes 6x Fusion of Full length ceRab2

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-214
    

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pET28-ceRab5(1-208)

    Plasmid

    #59551

    Purpose

    makes 6x Fusion of Full length ceRab5

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-208
    

    Tags

    6x His

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pET28-ceRab10(1-201)

    Plasmid

    #59552

    Purpose

    makes 6x Fusion of Full length ceRab10

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-208
    

    Tags

    6x His

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pET28-ceRab11(1-211)

    Plasmid

    #59553

    Purpose

    makes 6x Fusion of Full length ceRab11

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-211
    

    Tags

    6x His

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pET28-ceRab21(1-207)

    Plasmid

    #59554

    Purpose

    makes 6x Fusion of Full length ceRab21

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-207
    

    Tags

    6x His

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pET28-ceRab21(1-209)

    Plasmid

    #59555

    Purpose

    makes 6x Fusion of Full length ceRab35

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-209
    

    Tags

    6x His

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pET-20b-T7-Ntx

    Plasmid

    #58714

    Purpose

    Expresses T7-Notexin in Escherichia coli

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Tags

    T7 peptide tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET14b-PITPalpha N90F

    Plasmid

    #58257

    Purpose

    bacterial expression of human PITP alpha N90F

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N90F (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pET14b-PITPalpha K61A

    Plasmid

    #58258

    Purpose

    bacterial expression of human PITP alpha K61A

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pET29b-ClbP-S95A

    Plasmid

    #48245

    Purpose

    Untagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbP
    

    Expression

    Bacterial

    Mutation

    Changed Serine 95 to Alanine

    Promoter

    T7

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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