We narrowed to 24,695 results for: lars

1. GFP-PODXL FBM*/PBM*

   Plasmid

   #195221

   Purpose

   Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.

   Depositor

   Keith Mostov, David Bryant

   Article

   Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

   Insert

   PODXL (PODXL Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Dual mutant for HRS>AAA / lacking DTHL

   Promoter

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRL-VcnTS-E1015A-E1021A

   Plasmid

   #213411

   Purpose

   Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Mutated vinculin glutamic acid 1015 and 1021 to a…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMMK ENTR 1 HITI-2c ACTB

   Plasmid

   #206269

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   HITI-2c donor
   

   Use

   CRISPR; Multimate/gateway entr 1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro

   Plasmid

   #177145

   Purpose

   Lentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX4T3-GST-NQO1(Y128F)

   Plasmid

   #177141

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   NQO1 (NQO1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX4T3-GST-PolB(L301R/V303R/V306R)

   Plasmid

   #177137

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(L301R/V303R/V306R)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX4T3-GST-PolB(K206A/K244A/T304I)

   Plasmid

   #177134

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/T304I) with a TEV protease site located between the GST tag and PolB(K206A/K244A/T304I)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK

   Plasmid

   #126651

   Purpose

   B6J Lrig1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   Lrig1 (Lrig1 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   C-terminal fusion of T2A-sfGFP-iCre-ERT2

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-beta2(616-951)Double

   Plasmid

   #100746

   Purpose

   Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Long isoform with deletion of clathrin binding mo…

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. GST-beta2(616-951)Y815A

    Plasmid

    #100744

    Purpose

    Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Long isoform with Y815A mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GST-beta2(616-951)∆CBM

    Plasmid

    #100745

    Purpose

    Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    beta2 adaptin (AP2B1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Long isoform with deletion of clathrin binding mo…

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV5b HA Irx5 deltaHD

    Plasmid

    #24997

    Depositor

    Benoit Bruneau

    Article

    Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

    Insert

    Irx5 (Irx5 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletes entire HD (aa 112-177)

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pLV-SspB-HaloTag-p63DH

    Plasmid

    #176129

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-PalmGRET

    Plasmid

    #158221

    Purpose

    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

    Depositor

    Charles P. Lai

    Article

    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

    Insert

    PalmGFP-Nluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3-EF1A-mTert

    Plasmid

    #162555

    Purpose

    Overexpression of Tert

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of Tert (Tert Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SSpB-HaloTag-p63DH

    Plasmid

    #176116

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cas9/pTREX-n

    Plasmid

    #68708

    Purpose

    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Insert

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    

    Use

    CRISPR

    Tags

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

    Expression

    Mutation

    C4239T mutation that eliminates a BamHI restricti…

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. SSpB-mCherry-p63DH

    Plasmid

    #176112

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-UBC-FLEX-XRI-FLAG

    Plasmid

    #178057

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-FLAG
    

    Use

    AAV

    Tags

    FLAG-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. IL2R/hE-cadherin-cytotail

    Plasmid

    #45773

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Interleukin-2 receptor α subunit extracellular an…

    Promoter

    CMV

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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