We narrowed to 15,155 results for: pET

1. pET28a-PKM2-R399E

   Plasmid

   #122691

   Purpose

   Express His-tagged PKM2 (R399E) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   R399E

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pet16b-Avi-ubiquitin_rbs_BirA

   Plasmid

   #134897

   Purpose

   Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Inserts

   Ubiquitin (UBB Human)
   BirA
   

   Tags

   10HIS-AviTag-PreScission(3C)protease

   Expression

   Bacterial

   Promoter

   RBS and T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-20b-M-Ntx-S1

   Plasmid

   #58716

   Purpose

   Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Changed Asparagine 1 to Serine

   Promoter

   T7

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET 2C-T10-dCasX

   Plasmid

   #126418

   Purpose

   E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   DpbCasX
   

   Use

   CRISPR

   Tags

   His-MBP

   Expression

   Bacterial

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETSUMO2_Bacteroidetes-bGSDM-TEV

   Plasmid

   #222101

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Bacteroidetes-bGSDM-TEV ORF
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   TEV protease site replaces residues E242–G248

   Promoter

   T7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21b-Caspase-3(V266E)

   Plasmid

   #90089

   Purpose

   Expresses caspase 3 V266E mutant in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

   Insert

   Caspase 3 (CASP3 Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   V266E

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30a-His6-LOXCATmut

   Plasmid

   #140085

   Purpose

   A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutation in H265A and R268A in LOX (used original…

   Promoter

   T7

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-PKM2-H391Y

   Plasmid

   #122692

   Purpose

   Express His-tagged PKM2 (H391Y) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   H391Y

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a HAT epitope

   Plasmid

   #129445

   Purpose

   expression of HAT epitope

   Depositor

   Oskar Laur

   Insert

   HAT epitope
   

   Expression

   Bacterial

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET24b-SUMO-BG2.4

    Plasmid

    #199438

    Purpose

    expresses BAmGreen2.4 in bacterial cells with a SUMO tag

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen2.4
    

    Tags

    his tag and sumo

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_6xHis-PARP1-ART

    Plasmid

    #174810

    Purpose

    Bacterial expression of isolated PARP1 ART subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-ART (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28_6xHis-PARP1-BRCT

    Plasmid

    #174807

    Purpose

    Bacterial expression of isolated PARP1 BRCT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-BRCT (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-WGR

    Plasmid

    #174808

    Purpose

    Bacterial expression of isolated PARP1 WGR domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-WGR (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET23b-Casp6-His

    Plasmid

    #11823

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 6 (CASP6 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-eGFP-MinD

    Plasmid

    #133622

    Purpose

    Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

    Depositor

    Petra Schwille

    Article

    Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

    Insert

    EGFP-MinD (minD E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET_MBP-RGG-RGG

    Plasmid

    #124946

    Purpose

    Expression of MBP-RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-6xHis-mCherry(-6)

    Plasmid

    #205045

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    mCherry(-6)
    

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. hPU.1ΔN165-pET28b

    Plasmid

    #199796

    Purpose

    Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

    Depositor

    Gregory Poon

    Article

    Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

    Insert

    spi1 (SPI1 Human)
    

    Tags

    None

    Expression

    Bacterial

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-SUMO-mGSDMD

    Plasmid

    #111560

    Purpose

    Express mouse GSDMD in E.coli

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Tags

    His, SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET14b – lambda exonuclease

    Plasmid

    #104531

    Purpose

    bacterial expression of lambda exonuclease

    Depositor

    Charles Bell

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

    Insert

    λ exonuclease
    

    Tags

    His6 followed by a thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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