We narrowed to 4,217 results for: Alk

1. pUC57 mini-FLAG-HA-Regnase-3 D252N

   Plasmid

   #222652

   Purpose

   For in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12c (Zc3h12c Mouse)
   

   Use

   In vitro transcription

   Tags

   FLAG and HA

   Expression

   Mutation

   mutated aspartic acid 252 to asparagine to abroga…

   Promoter

   T7

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT

   Plasmid

   #222649

   Purpose

   For in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12a (Zc3h12a Mouse)
   

   Use

   In vitro transcription

   Tags

   FLAG and HA

   Expression

   Mutation

   Promoter

   T7

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK5664

   Plasmid

   #219747

   Purpose

   DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pCMV - nnLuz_v4 - tSV40
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNK291

   Plasmid

   #219748

   Purpose

   DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of CMV promoter, for mammalian expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pCMV - nnH3H_v2 - tSV40
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-TRE-V5-TurboID-linker-MUTT2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218911

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-MUTT2A-huGR/BlastR (NR3C1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 4155C>T and 4729T>G and 4731…

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLC047 - pL2_p2x35s-CrGLK-tMAS

   Plasmid

   #203906

   Purpose

   Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   CrGLK
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Silent mutation of BpiI or BsaI cut site for MoCl…

   Promoter

   CaMV 2x35S

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB278 - pL0_pT16H2 (pro + 5U)

   Plasmid

   #203889

   Purpose

   T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   T16H2 promoter and 5'UTR
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Mutation of BpiI or BsaI cut site for MoClo domes…

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUN1224 - pL0_pT3R (pro + 5U)

   Plasmid

   #203892

   Purpose

   T3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   T3R promoter and 5'UTR
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Mutation of BpiI or BsaI cut site for MoClo domes…

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUN1215 - pL0_pDAT (pro + 5U)

   Plasmid

   #203895

   Purpose

   DAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   DAT promoter and 5'UTR
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Mutation of BpiI or BsaI cut site for MoClo domes…

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSB55 - pL0_CrGLK (CDS)

    Plasmid

    #203903

    Purpose

    MoClo compatible L0 plasmid containing the CrGLK coding sequence

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    CrGLK
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Silent mutation of BpiI or BsaI cut site for MoCl…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)Gα12-133-NLuc

    Plasmid

    #207156

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNA12 (GNA12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position A(133)F

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ACVR1-TASA-R206H

    Plasmid

    #206182

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    S190A, S194A, R206H

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ACVR1-TSAA-R206H

    Plasmid

    #206183

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S192A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    S192A, S194A, R206H

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ACVR1-VSAS-R206H

    Plasmid

    #206185

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S192A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    T189V, S192A, R206H

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ACVR1-VASS-R206H

    Plasmid

    #206186

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S190A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    T189V, S190A, R206H

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV1-DIO-eGFP-pvRPL10a

    Plasmid

    #202542

    Purpose

    Cr-dependent (DIO) expression of eGFP-tagged ribosomal subunit that uses the prairie vole RPL10a gene sequence under the human synapsin promoter.

    Depositor

    Zoe Donaldson

    Article

    Sadino et al Elife. 2023 Feb 28;12:e80517. doi: 10.75

    Insert

    RPL10a subunit of the ribosome
    

    Use

    AAV and Cre/Lox

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    optimized for prairie vole DNA sequence and optim…

    Promoter

    human synapsin

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBactin-Nedd1-mOrange2

    Plasmid

    #196860

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2 (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

    Plasmid

    #196874

    Purpose

    Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynGFP
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP

    Plasmid

    #196876

    Purpose

    Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynEGFP
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

    Plasmid

    #196883

    Purpose

    Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-ZsGreen-bGH
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge