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slc1a3b:myrGFP-P2A-H2AmCherry

Plasmid

#170208

Purpose

Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

Depositor

Kelly Monk

Publication

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

slc1a3b (slc1a3b Zebrafish)

Use

Zebrafish

Tags

myrGFP-P2A-H2AmCherry

Promoter

slc1a3b

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED

Plasmid

#154088

Purpose

Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.

Depositor

George Daley

Publication

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Ligand Dependent Nuclear Receptor Corepressor, HOXA9, HOXA5 (LCOR, HOXA9, HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166013

Purpose

mammalian expression of MERS-CoV soluble spike trimer protein

Depositor

Dominic Esposito

Publication

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

MERS-CoV S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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PL-5LTR-RGR(DMD#1)-AmCyan-A

Plasmid

#138482

Purpose

Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

Depositor

Akitsu Hotta

Publication

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ribozyme-flanked gRNA and AmCyan

Use

CRISPR

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX305_FKBP12F36V-SHOC2

Plasmid

#134522

Purpose

Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

Depositor

Andrew Aguirre

Publication

Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Tags

FKBP12F36V-2xHA

Expression

Mammalian

Mutation

Silent mutations made at wobble base position at …

Promoter

hPGK

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-BCL2

Plasmid

#116717

Purpose

Lentiviral expression of BCL2

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BCL2 (BCL2 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/TO-3xFLAG-REV7

Plasmid

#114127

Purpose

Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

Depositor

Daniel Durocher

Publication

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

REV7 (MAD2L2 Human)

Use

Genomic integration, tetracycline inducible

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pcDNA5FRT/TO-Npl4-Strep-HA

Plasmid

#113495

Purpose

Expression of human Npl4 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Publication

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Npl4 (NPLOC4 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEF5-FRT-V5-DEST-GFP-Kif26b

Plasmid

#102862

Purpose

Expresses GFP-Kif26b in mammalian cells

Depositor

Henry Ho

Publication

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mouse Kif26b (Kif26b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

EF1A

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSAM2_mCherry_Gata4

Plasmid

#72690

Purpose

Doxycyline regulated expression of Gata4 in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 4 (Gata4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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IQGAP1_pLX307

Plasmid

#98346

Purpose

Lentiviral expression of IQGAP1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

IQGAP1 (IQGAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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sox10:mCherry

Plasmid

#98695

Purpose

I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

Depositor

Thomas Look

Publication

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

mCherry

Use

Zebrafish expression

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLgw Cbx1-V5-EcoDam

Plasmid

#59211

Purpose

Mammalian DamID positive control lentiviral plasmid. Expresses Cbx1-V5-Dam.

Depositor

Bas van Steensel

Publication

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Insert

Cbx1 (Cbx1 Mouse)

Use

Lentiviral; Damid

Tags

Dam and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter and heat shock minima…

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]

Plasmid

#66753

Purpose

miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondria

Depositor

Yishi Jin

Publication

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

tomm-20 (N terminal 55 aa)

Expression

Worm

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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DBDmut-CA-RIT-NFAT1

Plasmid

#63669

Purpose

Constitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding

Depositor

Anjana Rao

Publication

Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

Insert

NFAT1 (Nfatc2 Mouse)

Use

Retroviral

Tags

3x HA

Expression

Mammalian

Mutation

CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pFRT-DestFLAGHA_RNAseH1

Plasmid

#65783

Purpose

constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells

Depositor

Thomas Tuschl

Insert

RNaseH1 (RNASEH1 Human, Zebrafish)

Tags

GFP

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDEST26 Ago2

Plasmid

#59904

Purpose

Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells

Depositor

Carl Novina

Publication

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1 _60-89

Plasmid

#59144

Purpose

YAP1 mutants that harbored a deletion of amino acide at 60-89 position, which disrupts binding to TEAD transcription factors

Depositor

William Hahn

Publication

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

aa60-89 deleted

Promoter

CMV

Available since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pSCT-GAL93-SFPQ

Plasmid

#46320

Depositor

Steven Brown

Publication

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

SFPQ (SFPQ Human)

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pT3-EFa1-HA-Jag1

Plasmid

#46051

Depositor

Xin Chen

Publication

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Jag1 (Jag1 Rat)

Tags

HA tag

Expression

Mammalian

Promoter

EF1a

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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