We narrowed to 4,972 results for: IRES

1. Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9

   Plasmid

   #231909

   Purpose

   For packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   EGFP-P2A-dTomato-IRES-Luc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP1666 - pAAV-AiE0486m-minBG-IRES2-iCre-HGHpA

   Plasmid

   #220710

   Purpose

   AiE0486m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

   Depositor

   Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

   Insert

   iCre
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry

   Plasmid

   #232246

   Purpose

   Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   delta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   delta73-82

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)

   Plasmid

   #231233

   Purpose

   MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MYC (MYC Human)
   

   Expression

   Mammalian

   Mutation

   TAD deleted (delta1-143); numbering based on sequ…

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP

   Plasmid

   #223253

   Purpose

   AAV construct for Cre-dependent (FLEX) expression of macaque melanopsin (Opn4, short isoform, GenBank:OP856641; hSyn promoter) and of EGFP via an IRES.

   Depositor

   Michael Do

   Article

   Liu et al Science. 2023 Jan 27;379(6630):376-381.

   Insert

   macaque OPN4-IRES-EGFP (OPN4 )
   

   Use

   AAV

   Promoter

   human Synapsin 1 (hSyn)

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry

   Plasmid

   #226104

   Purpose

   Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   COX8A (COX8A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   encodes only for mitochondrial targeting sequence…

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcs2-cDELE1(142-515)-GFP-IRES-mCherry

   Plasmid

   #226102

   Purpose

   cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   DELE1 (DELE1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   only encodes for residues 142-515 of DELE1 corres…

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP

   Plasmid

   #225490

   Purpose

   Neuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   Neuronal expression

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

   Plasmid

   #224532

   Purpose

   A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-H2A-mCherry-SV40pA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXS-IRES-BLAST flag-CAD H1734A H1741A

    Plasmid

    #169830

    Purpose

    Expresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    H1734A H1741A; TCCC -> AGTC silent mutations a…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXS-IRES-BLAST flag-CAD delta Loop1

    Plasmid

    #169831

    Purpose

    Expresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids F1366 - P1376; TCCC ->…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-BLAST flag-CAD delta Loop2

    Plasmid

    #169832

    Purpose

    Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids R1398 - S1407; TCCC ->…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXS-IRES-BLAST flag-CAD T456A S1406A

    Plasmid

    #169823

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1406A; TCCC -> AGTC silent mutations at…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXS-IRES-BLAST flag-CAD T456A S1859A

    Plasmid

    #169824

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1859A; TCCC -> AGTC silent mutations at…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #218637

    Purpose

    Proximity biotinylation control of OSBP(PH domain)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    OSBP (OSBP Human)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    PH domain only

    Promoter

    UBC

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

    Plasmid

    #207171

    Purpose

    expression plasmid for Gal4DBD-HIF-CAD.myc expression

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    Gal4DBD-HIF1a-CAD Myc tag linker
    

    Tags

    2xMyc tag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

    Plasmid

    #202090

    Purpose

    Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

    Depositor

    Elizabeth Heller

    Article

    Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

    Insert

    dCas9-Set2(R195G, C201A)
    

    Tags

    3x FLAG and mCherry

    Expression

    Mammalian

    Mutation

    R195G and C201A abolish catalytic activity

    Promoter

    human Synapsin

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-IRES-BLAST flag-CAD R1403A R1404A

    Plasmid

    #188135

    Purpose

    Expresses C-terminal flag-tagged human CAD R1403A R1404A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXS-IRES-BLAST flag-CAD E1367A E1368A

    Plasmid

    #188143

    Purpose

    Expresses C-terminal flag-tagged human CAD E1367A E1368A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXS-IRES-BLAST flag-CAD P1375A P1376A

    Plasmid

    #188146

    Purpose

    Expresses C-terminal flag-tagged human CAD P1375A P1376A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge