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Naturally Occurring Off-Switches for CRISPR-Cas9.
Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR
Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85677pEJS427-pCSDest2-AcrE2Mammalian expression of anti-CRISPR protein from Type I-E
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85678pEJS430-pCSDest2-AcrIIC1BoeMammalian expression of Type II-C anti-CRISPR protein AcrIIC1Boe from Brackiella oedipodis
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85679pEJS433-pCSDest2-AcrIIC1NmeMammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidis
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85712pEJS436-pCSDest2-AcrIIC2NmeMammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidis
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85713pEJS443-pCSDest2-AcrIIC3NmeMammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidis
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85714pEJS468-pLK.O1-NmeSgRNA/DTS13-TelomereTelomere-targeting Nme sgRNA under U6 promoter
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85715pEJS469-pLK.O1-SpySgRNA/DTS13-TelomereTelomere-targeting Spy sgRNA under U6 promoter
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85716pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-oneExpresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA
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85717pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-oneExpresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA
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85748pEJS507-pCDest2-noAcr-mTagBFP2-IRESExpresses mTagBFP2 only; used as a control
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85749pEJS481-pCDest2-AcrE2-mTagBFP2-IRESExpresses anti-CRISPR protein from Type I-E and mTagBFP2
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85750pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRESExpresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2
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87448pCSDest2-NmeCas9-NLS-3XHA-NLSMammalian expression of wild type Nme Cas9
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