We narrowed to 8,170 results for: ara-2

1. PHR-GFP-Rta

   Plasmid

   #183930

   Purpose

   Optogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   PHR-GFP-Rta (CRY2 Mustard Weed, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PHR-GFP-VPR

   Plasmid

   #183928

   Purpose

   Optogenetic PHR domain coupled to GFP and VPR activation domain; binds to CIBN upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   PHR-GFP-VPR (CRY2 Mustard Weed, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. TET1CD-CRY2-EGFP

   Plasmid

   #82555

   Purpose

   Encodes TET1 under LITE2.0 system

   Depositor

   Joseph Irudayaraj

   Article

   Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

   Inserts

   TET1 (TET1 Human)
   CRY2 (CRY2 Mustard Weed)
   EGFP
   

   Use

   AAV

   Tags

   2A, NLS(alpha-imp), and NLS-VP64

   Expression

   Mammalian

   Mutation

   CRY2PHR (N-terminal photolyase homology region do…

   Promoter

   EF-1alpha

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR207-MUM2sp-ADPG2

   Plasmid

   #170723

   Purpose

   Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

   Depositor

   George Haughn

   Article

   McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

   Insert

   ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
   

   Use

   Gateway donor vector / entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. PhyB-mCherry-Tom7

   Plasmid

   #66571

   Purpose

   Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae

   Depositor

   Orion Weiner

   Article

   Jost et al ACS Synth Biol. 2015 Jun 2.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   Tom7 and mCherry

   Expression

   Yeast

   Mutation

   PhyB portion contains aa's 1-908

   Promoter

   pADH1

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPO640

   Plasmid

   #195296

   Purpose

   pTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   miniMcCAST (Tn7575) with KanR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTet-Bxb1-Xis-mScarlet

   Plasmid

   #195570

   Purpose

   Expresses Bxb1 excisionase fused with mScarlet for fluorescence

   Depositor

   Richard Murray

   Article

   Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

   Insert

   Bxb1 Excisionase Fused With mScarlet
   

   Use

   Synthetic Biology

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   P7

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTet-Bxb1-CFP

   Plasmid

   #195374

   Purpose

   Expresses Bxb1 integrase fused with CFP for fluorescence

   Depositor

   Richard Murray

   Article

   Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

   Insert

   Bxb1 Integrase Fused With deCFP
   

   Use

   Synthetic Biology

   Tags

   deCFP

   Expression

   Mutation

   Promoter

   pTet

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-MICU1-mEF

   Plasmid

   #31765

   Depositor

   Vamsi Mootha

   Article

   Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

   Insert

   MICU1 (MICU1 Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   Mutated in both EF-hand domains: D231A, E242K, D4…

   Promoter

   Available since

   May 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR-221-P3-P4

    Plasmid

    #186351

    Purpose

    Gateway donor vector with AttP3 AttP4 recombination sites

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR-221-P3-P5

    Plasmid

    #186350

    Purpose

    Gateway donor vector with AttP3 AttP5 recombination sites

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEEV2/11

    Plasmid

    #166888

    Purpose

    Helper/packaging plasmid for production of AAV2/11 pseudotype vector

    Depositor

    Tadahito Kanda, Seiichiro Mori

    Article

    Mori et al Virology. 2004 Dec 20;330(2):375-83. doi

    Insert

    AAV2 rep/AAV11 cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    AAV2 p5

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETduet+sfGFP-TetR

    Plasmid

    #157794

    Purpose

    Expression of sfGFP-TetR fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET19b+H3

    Plasmid

    #157807

    Purpose

    Expression of cysteine-free human histone H3 (c111A)

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C111A

    Promoter

    T7

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBTR(hCc)

    Plasmid

    #61026

    Purpose

    Expresses horse holocytochrome c in E. coli

    Depositor

    Gary Pielak

    Article

    Patel et al Protein Expr Purif. 2001 Jul;22(2):220-4

    Insert

    horse cytochrome c (CYCS Equus*)
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    *see comments

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGWB660-CYCD3;1

    Plasmid

    #140751

    Purpose

    Inducing plant cells to enter mitosis

    Depositor

    Bo Liu

    Article

    Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

    Insert

    Cyclin D3;1 (CYCD3;1 Mustard Weed)
    

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35 promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. YSBE 610 : pHYG_6xHA

    Plasmid

    #92091

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    6xHA
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC_sgRNA

    Plasmid

    #68710

    Purpose

    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCL.140

    Plasmid

    #184995

    Purpose

    Express Cas9 in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TetOn-3g

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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