We narrowed to 15,139 results for: nans

1. (SUMO)10-(SIM)5

   Plasmid

   #126866

   Purpose

   Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-WT (AF41)

   Plasmid

   #21367

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Use

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   wild-type sequence*

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet

   Plasmid

   #130708

   Purpose

   Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   NTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet
   

   Use

   Tags

   NTOM20 and SNAP-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-MYRIP

   Plasmid

   #89586

   Purpose

   Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYRIP (Myrip Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-GGG-HIS-CBM-Xmod-DocIII

   Plasmid

   #91698

   Purpose

   CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-HIS-CBM-Xmod-DocIII
   

   Use

   Tags

   HIS-Tag and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Cysteine deletion in CBM C63S

   Promoter

   T7

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Syn-pAceR

   Plasmid

   #195521

   Purpose

   Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAceR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   VARNAM 78E, 81D, 92N; SI linker

   Promoter

   Synapsin

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ALC1-c007

   Plasmid

   #173112

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   CHD1L (CHD1L Human)
   

   Use

   Tags

   Nanoluc

   Expression

   Bacterial

   Mutation

   L613-K879, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC841 - pAAV minP secNLuc

   Plasmid

   #82498

   Purpose

   Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

   Depositor

   Brandon Harvey

   Article

   Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

   Insert

   secNLuc
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   c-Fos minimal promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P-1-eIF4E K119A

   Plasmid

   #199440

   Purpose

   Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

   Depositor

   Shin-ichi Hoshino

   Article

   Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

   Insert

   1977 (EIF4E Human)
   

   Use

   Tags

   GST-fusion protein

   Expression

   Bacterial

   Mutation

   changed lysine 119 to alanine

   Promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-2xSV40_NLS-NLuc

    Plasmid

    #135953

    Purpose

    Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe puro RalA S31N

    Plasmid

    #19718

    Depositor

    Channing Der

    Article

    Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

    Insert

    V-ral simian leukemia viral oncogene homolog A (RALA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S31N mutant for dominant neg function. NOTE: lo…

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pROF402

    Plasmid

    #155334

    Purpose

    Binary vector for expression of FLuc controlled by the blue light-repressible system 

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB
    

    Use

    Luciferase and Synthetic Biology; Eukaryotic expr…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pROF441

    Plasmid

    #155336

    Purpose

    Binary vector for expression of a gRNA targeting AtAP1 promoter 

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. J-HARS-4

    Plasmid

    #166529

    Purpose

    scFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. L-SARS-7

    Plasmid

    #166527

    Purpose

    scFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. L-RARS-5

    Plasmid

    #166535

    Purpose

    scFv of a human scaffold targeting Arginyl-tRNA synthetase. Antigen coverage aa 70-660 of 660

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L-KARS-5

    Plasmid

    #166530

    Purpose

    scFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. O-DARS-1

    Plasmid

    #166538

    Purpose

    scFv of a human scaffold targeting Aspartyl-tRNA synthetase. Antigen coverage aa 1-501 of 501, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. O-LARS-15

    Plasmid

    #166542

    Purpose

    scFv of a human scaffold targeting Leucyl-tRNA synthetase. Antigen coverage aa 260-509 of 1176

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. B2387_mEGFP-FKBP2x

    Plasmid

    #216216

    Purpose

    Expresses mEGFP(A206K) fused to FKBP-FKBP in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    mEGFP-FKBP-FKBP (FKBP1A Human)
    

    Use

    Tags

    EGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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