We narrowed to 6,254 results for: SPI

1. pET24a-VHH-myc

   Plasmid

   #109422

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-PRDM16 880-1038

   Plasmid

   #53350

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Shingo Kajimura, Bruce Spiegelman

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 880-1038

   Promoter

   tac

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. GST-PRDM16 224-454

   Plasmid

   #53347

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Shingo Kajimura, Bruce Spiegelman

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 224-454

   Promoter

   tac

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCMX-PGC1b

   Plasmid

   #1032

   Depositor

   Bruce Spiegelman

   Article

   Lin et al J Biol Chem 2002 Jan 18;277(3):1645-8. E

   Insert

   PGC-1 beta (Ppargc1b Mouse)
   

   Use

   Tags

   Gal4

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

5. GST-PRDM16 1-223

   Plasmid

   #53346

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Shingo Kajimura, Bruce Spiegelman

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 1-223

   Promoter

   tac

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. Gal4 PGC1 alpha 1-550

   Plasmid

   #8894

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   Gal4 DBD

   Expression

   Mammalian

   Mutation

   amino acids 1-550 of PGC-1 alpha

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

7. GST-PRDM16 1039-1176

   Plasmid

   #53351

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Shingo Kajimura, Bruce Spiegelman

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 1039-1176

   Promoter

   tac

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. GST-PRDM16 681-880

   Plasmid

   #53349

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Shingo Kajimura, Bruce Spiegelman

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 681-880

   Promoter

   tac

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. GST-PRDM16 455-680

   Plasmid

   #53348

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Shingo Kajimura, Bruce Spiegelman

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 455-680

   Promoter

   tac

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. ANKRD24-TwinStrepII

    Plasmid

    #83190

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Article

    Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

    Insert

    ANKRD24 (Ankrd24 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET24a-VHH-tev-APEX2

    Plasmid

    #117751

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-f:PGC1(delta CTD)

    Plasmid

    #1030

    Depositor

    Bruce Spiegelman

    Article

    Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

    Insert

    PGC1a (Ppargc1a Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta CTD

    Promoter

    Available since

    Oct. 4, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Gal4 PGC1 alpha 1-350

    Plasmid

    #8893

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Gal4 DBD

    Expression

    Mammalian

    Mutation

    amino acids 1-350 of PGC-1 alpha

    Promoter

    Available since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pET24a-LaM4-1xTS

    Plasmid

    #162778

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and TS site from proCCK

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-PGC1(1-180)

    Plasmid

    #1028

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains only aa1-180 (the EcoRI site)

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

    Plasmid

    #229827

    Purpose

    A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    3xnls-TagBFP2-MCS-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

    Plasmid

    #229807

    Purpose

    A CAGGS driven tet-on transactivator with an nls-EGFP reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    rtTA-IRES-nls-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGEN-Dre-nls (JDW 1131)

    Plasmid

    #229809

    Purpose

    A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    DRE recombinase-nls
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

    Plasmid

    #229819

    Purpose

    A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

    Plasmid

    #229837

    Purpose

    A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge