We narrowed to 62 results for: 4G cloning

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sfGFP-SasG(4G)-mCherry

Plasmid

#206946

Purpose

Mammalian expression of heterobifunctional SasG linkers (4G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (4G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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4G-Cloning Plasmid Kit

Plasmid Kit

#1000000252

Purpose

Use 4G (Golden-Gate-guided Gibson) cloning for rapid assembly of single- or multi-gene expression vectors for multi-subunit protein complexes in various expression hosts.

Depositor

Stephan Gruber

Application

Cloning and Synthetic Biology

Vector Type

Bacterial Expression

Cloning Type

Golden Gate

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only
pD(Ct)_FLAG3

Plasmid

#223896

Purpose

4G-cloning donor for C-terminal FLAG3 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

FLAG3

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMulti_S_ccdB

Plasmid

#223853

Purpose

GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Type

Empty backbone

Use

Golden-gate acceptor

Expression

Bacterial

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMulti_K_ccdB

Plasmid

#223852

Purpose

GEC-acceptor for 4G-cloning, designed for expression in E. coli (Kanamyicin resistance)

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Type

Empty backbone

Use

Golden-gate acceptor

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMulti_A_ccdB

Plasmid

#223851

Purpose

GEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Type

Empty backbone

Use

Golden-gate acceptor

Expression

Bacterial

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_MYC9

Plasmid

#223897

Purpose

4G-cloning donor for C-terminal MYC9 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

Myc9

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_PK6

Plasmid

#223898

Purpose

4G-cloning donor for C-terminal PK6 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

PK6

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG

Plasmid

#223895

Purpose

4G-cloning donor for C-terminal FLAG tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

FLAG

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_HALO

Plasmid

#223899

Purpose

4G-cloning donor for C-terminal HALO tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

HALO

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_HALO

Plasmid

#223885

Purpose

4G-cloning donor for N-terminal HALO tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

HALO

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_GFP

Plasmid

#223880

Purpose

4G-cloning donor for N-terminal GFP tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

GFP

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_His8

Plasmid

#223886

Purpose

4G-cloning donor for C-terminal His8 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His8

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_TS

Plasmid

#223891

Purpose

4G-cloning donor for C-terminal TwinStrep tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

TwinStrep

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_CMVN

Plasmid

#223861

Purpose

4G-cloning donor for mammalian promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

CMV promoter and bGH polyA

Use

Golden-gate donor

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_phN

Plasmid

#223858

Purpose

4G-cloning donor for insect promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-TS-His10

Plasmid

#223893

Purpose

4G-cloning donor for C-terminal 3C-TwinSTrep-His10 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3C-TwinStrep-His10

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_Avi-3C-TS

Plasmid

#223894

Purpose

4G-cloning donor for C-terminal AviTag-3C-TwinStrep tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

AviTag-3C-TwinStrep

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-GST-TEV

Plasmid

#223878

Purpose

4G-cloning donor for N-terminal His6-GST-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-GST-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-MBP-TEV

Plasmid

#223877

Purpose

4G-cloning donor for N-terminal His6-MBP-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-MBP-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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