We narrowed to 837 results for: Anti-CRISPR

1. dCas9 fused to scFv (anti-spike)

   Plasmid

   #186421

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-scFv fusion (anti-SARS-CoV-2 spike)
   

   Use

   CRISPR

   Tags

   6His

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA giantin/GOLGB1

   Plasmid

   #222315

   Purpose

   CRISPR/Cas9 close to the ATG of giantin/GOLGB1 gene.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

   Insert

   gRNA targeting GOLB1 (GOLGB1 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9 fused to nanobody (anti-beta catenin)

   Plasmid

   #186420

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-nanobody fusion (anti-beta catenin)
   

   Use

   CRISPR

   Tags

   6His

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_PGK_antiCD19_H1_Gal4VP64

   Plasmid

   #169918

   Purpose

   Expression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.

   Depositor

   Zhili Rong

   Article

   Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

   Insert

   antiCD19-Notch1-GL4VP64
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_PGK_antiCD19_Z3_Gal4VP64

   Plasmid

   #169916

   Purpose

   Expression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.

   Depositor

   Zhili Rong

   Article

   Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

   Insert

   antiCD19-Notch3-GL4VP64
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-giantin-ATG_donor

   Plasmid

   #222316

   Purpose

   Perform knock-in by homologous recombination in giantin/GOLGB1 gene.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

   Insert

   EGFP and homology arms for giantin/GOLGB1
   

   Use

   CRISPR

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_PGK_antiCD19_eZ3_Gal4VP64

   Plasmid

   #169917

   Purpose

   Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

   Depositor

   Zhili Rong

   Article

   Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

   Insert

   antiCD19-Notch3-GL4VP64
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Additional EGF repeat between the extracellular d…

   Promoter

   PGK

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Jun lab tunable CRISPRi strain

   Bacterial Strain

   #86400

   Purpose

   Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

   Depositor

   Suckjoon Jun

   Article

   Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

   Bacterial Resistance

   Spectinomycin and hygromycin

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

   Plasmid

   #66707

   Purpose

   expresses a single gRNA and VP64-dCas9-VP64

   Depositor

   Charles Gersbach

   Inserts

   hU6-gRNA expression cassette
   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Mutation

   D10A and H840A

   Promoter

   hU6 and hUbC

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJET-41-DExCon-antiGFPnanobody-mCherry-R11b

    Plasmid

    #179901

    Purpose

    Donor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry module

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    antiGFPnanobody-mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3GS

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRL-gRNA(anti-cGAS)-Cas9-Puro

    Plasmid

    #130908

    Purpose

    gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    Cas9
    

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJET-42-DExCon-antiGFPnanobody-mCherry-R11a

    Plasmid

    #179902

    Purpose

    Donor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry module

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    antiGFPnanobody-mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3GS

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223 SCAF8_isoD_CRISPR_resistant

    Plasmid

    #122468

    Purpose

    pDONR for Gateway cloning

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF8 isoform D CRISPR resistant (SCAF8 Human)
    

    Use

    Gateway cloning

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223 SCAF4_CRISPR resistant

    Plasmid

    #122465

    Purpose

    pDONR for Gateway cloning

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF4 CRISPR resistant (SCAF4 Human)
    

    Use

    Gateway cloning

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223 SCAF8_isoC_CRISPR resistant

    Plasmid

    #122466

    Purpose

    pDONR for Gateway cloning

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF8 isoform C CRISPR resistant (SCAF8 Human)
    

    Use

    Gateway cloning

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT/TO/GFP-SCAF8 (CRISPR_resistant)

    Plasmid

    #122472

    Purpose

    Dox-inducible GFP (N-terminal tag) SCAF8 expression

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF8 (SCAF8 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    CMV

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFRT/TO/GFP-SCAF4 (CRISPR_resistant)

    Plasmid

    #122471

    Purpose

    Dox-inducible GFP (N-terminal tag) SCAF4 expression

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF4 (SCAF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    CMV

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CRISPRv2-sgNTC13-hygro

    Plasmid

    #231988

    Purpose

    Knockout non-targeting control

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CRISPRv2-sgNTC49-puro

    Plasmid

    #231989

    Purpose

    Knockout non-targeting control

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiCRISPR v2 puro hGSDME gRNA1

    Plasmid

    #223519

    Purpose

    knocking out hGSDME in human cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (GSDME Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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