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Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

Plasmid

#81253

Purpose

Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

TetR and LacI

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

Plasmid

#81251

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR

Plasmid

#82024

Purpose

Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

mKate2-2xNLS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

Plasmid

#81255

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 9 - U6p_30nt_stgRNA

Plasmid

#81248

Purpose

Expresses 30nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 11 - U6p_70nt_stgRNA

Plasmid

#81250

Purpose

Expresses 70nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 10 - U6p_40nt_stgRNA

Plasmid

#81249

Purpose

Expresses 40nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 31 - 1xTetO_H1p_20nt2_stgRNA_3xLacO_U6p_20nt3_stgRNA

Plasmid

#81254

Purpose

For expressing Dox and IPTG inducible stgRNAs

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

stgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 1 - U6p_20nt1_WT_sgRNA

Plasmid

#81245

Purpose

For assaying self-targeting activity via Cas9

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only

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Construct 13 - CMVp_U6p_27nt1_GFP(+3)_RFP(+2)

Plasmid

#81252

Purpose

Mutation based toggling reporter (MTBR)

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Mutation Based Toggling Reporter (MBTR)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA)

Plasmid

#81246

Purpose

For assaying self-targeting activity via Cas9

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

Plasmid

#81253

Purpose

Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

TetR and LacI

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

Plasmid

#81251

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR

Plasmid

#82024

Purpose

Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

mKate2-2xNLS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

Plasmid

#81255

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 9 - U6p_30nt_stgRNA

Plasmid

#81248

Purpose

Expresses 30nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 11 - U6p_70nt_stgRNA

Plasmid

#81250

Purpose

Expresses 70nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 10 - U6p_40nt_stgRNA

Plasmid

#81249

Purpose

Expresses 40nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 31 - 1xTetO_H1p_20nt2_stgRNA_3xLacO_U6p_20nt3_stgRNA

Plasmid

#81254

Purpose

For expressing Dox and IPTG inducible stgRNAs

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

stgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 1 - U6p_20nt1_WT_sgRNA

Plasmid

#81245

Purpose

For assaying self-targeting activity via Cas9

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge