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Plasmid Kit#1000000024PurposeUsed to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.DepositorAvailable sinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only
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biGBac Kit
Plasmid Kit#1000000088PurposeGeneration of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.DepositorAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Yeast Secrete and Detect
Plasmid Kit#1000000166PurposeYeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.DepositorAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
GreenGate 2.0 Toolkit
Plasmid Kit#1000000241PurposeThe GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.DepositorAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Kit
Plasmid Kit#1000000080PurposeGolden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit designDepositorAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human Kinase Domain Constructs for Automated Bacterial Expression
Plasmid Kit#1000000094Purpose68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expressionDepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Minimal Antibiotic Resistance Platform (ARP)
Plasmid Kit#1000000143PurposeUsed for the general dereplication of antibiotics in natural product extracts. Plasmids constitutively express individual resistance genes that target the most commonly found antibiotics.DepositorAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Chromoprotein kit 1.0
Plasmid Kit#1000000174PurposeA synthetic biology collection of plasmids for expression of chromoproteins in bacteria, which can be used as an educational resource.DepositorAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
The Expanded EasyClone-MarkerFree toolkit
Plasmid Kit#1000000190PurposeThe Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.DepositorAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CIDAR MoClo Extension, Volume I
Plasmid Kit#1000000161PurposeThis kit contains 110 plasmids designed to quickly and efficiently build a wide variety of gene expression constructs using the CIDAR-standard MoClo system.DepositorAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Platinum Gate TALEN Kit
Plasmid Kit#1000000043PurposeEnables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.DepositorAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
PermaPhos Kit
Plasmid Kit#1000000226PurposeThis kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression systemDepositorAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TALE Toolbox
Plasmid Kit#1000000019PurposeProvides a complete set of plasmids for building your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.DepositorAvailable sinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Phosphoinositide Biosensors Kit
Plasmid Kit#1000000261PurposeUse the phosphoinositide biosensors kit to detect the whole family of phosphoinositides in fixed cells and tissues.DepositorAvailable sinceJuly 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
Chromoprotein kit 2.0
Plasmid Kit#1000000198PurposeChromoprotein plasmid kit that contain two new chromoprotein genes that are less toxic to E. coli so that the plasmids are more stable during growth.DepositorAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Fragmid
Plasmid Kit#1000000237PurposeFragmid is a modular toolkit that leverages Golden Gate cloning to enable the rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.DepositorAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Nuclear Pro-Code Vector Kit
Plasmid Kit#1000000197PurposeThe Nuclear Pro-Code Vector Kit is a set of 75 lentiviral vector plasmids, each with a unique barcode (Pro-Code) and gRNA cloning site.DepositorAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
EasyClone 2.0 Yeast Toolkit
Plasmid Kit#1000000073PurposeIntegrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strainsDepositorAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
GoldenPiCS Kit
Plasmid Kit#1000000133PurposeGoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sitesDepositorAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-itis Bacterial Base Editor Activity
Plasmid Kit#1000000219PurposeThis collection contains all plasmids needed for preparation and execution of the "GFP-itis" bacterial base editing activity.DepositorAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only