33,449 results
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Plasmid#182500PurposeBacterial expression of the Homo sapiens calmodulin, CALM1DepositorAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pBAD-HisD-mNG-Ec-Kbp-D9N
Plasmid#178011PurposeBacterial expression of green fluorescent potassium indicator mNG-Ec-Kbp-D9N (KRaION1 mutant D9N)DepositorInsertmNG-Ec-Kbp-D9N
ExpressionBacterialPromoteraraBAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGJJ001
Plasmid#183619PurposebindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-Caspase-4_enzymatic-domain
Plasmid#183381PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET24a-LaM4-EGFP
Plasmid#182641PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
TagsBAP, EGFP, HA, His6, and T7ExpressionBacterialPromoterT7Available sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKOR1-mcs
Plasmid#181755PurposeAllele replacement for Staphylococcus aureusDepositorTypeEmpty backboneExpressionBacterialAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-SpCas9mSA-His
Plasmid#182951PurposeExpress SpCas9mSA in bacteriaDepositorInsertNLS-SpCas9mSA-His
UseCRISPRExpressionBacterialAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)
Plasmid#181792PurposeExpresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_EIF4E_eiF4E
Plasmid#178474PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_20bpLinker
Plasmid#157787PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_20bp linker
ExpressionBacterialAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB2_mScarletI_mVenusNB-MrkH
Plasmid#182291Purposeexpresses c-di-GMP biosensor in E. coliDepositorInsertmVenusNB-MrkH
TagsmVenusNB internal fusion to MrkHExpressionBacterialPromoterproCAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAT-WT-HA-GFP11
Plasmid#177719PurposeER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFPDepositorAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterENO1pAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSUMO His6 SUMO MYC3
Plasmid#177848PurposeBacterial Expression of MYC3DepositorAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCCM’
Plasmid#162709PurposepCCM reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationp15A origin replaced with high-copy colE1 originPromoterPLtet0-1 promoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7C-BAG2
Plasmid#177654PurposeRecombinant protein synthesis of human BAG2DepositorAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB225
Plasmid#170015PurposeEncodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporterDepositorPublicationInsertsmNeonGreen
Peptide display protein with no C-terminal peptide
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDule2-Acd82
Plasmid#174100PurposeAcridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertAcridone 82 tRNA synthetase and tRNA
ExpressionBacterialMutationN311S, C313G, V366A, W382TAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PtxS1-c003
Plasmid#173076PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPtxS1
ExpressionBacterialMutationD35-I221Available sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyExpressionBacterial and YeastAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only