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34,228 results

Showing: 4781 - 4800 of 34228 results

  9. pVP144

    Plasmid
    #222081
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.
    Depositor
    Wayne Parrott
    Publication
    Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi
    Insert
    dCas9-PmCDA1, sfGFP, sacB and eforRED
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    222081-plasmid-map-sequence-id-438346

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  14. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid
    #218509
    Purpose
    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination
    Depositor
    Emmanuelle Charpentier
    Publication
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mKate2
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Deletion of Plac_mrfp cassette, introduction of a…
    Promoter
    P23
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218509-plasmid-map-sequence-id-438730

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  15. pRSF-G1(4/5FTrp)RS

    Plasmid
    #207620
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Publication
    Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021
    Insert
    tRNA synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    207620-plasmid-map-sequence-id-435603

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  18. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Publication
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Expression
    Bacterial and Mammalian
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207412-plasmid-map-sequence-id-422689

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  19. pGR596

    Plasmid
    #213787
    Purpose
    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BadBoy3 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    See manuscript
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213787-plasmid-map-sequence-id-424625

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  20. SPLICSFAST-ER-MT-P2A

    Plasmid
    #214892
    Purpose
    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPLICSFAST-ER-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214892-plasmid-map-sequence-id-423478

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Showing: 4781 - 4800 of 34228 results