32,870 results
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Plasmid#109905PurposeProtein expression and purification of YWHAG_14-3-3DepositorInsertYWHAG_14-3-3 (YWHAG Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dual opposing T7 promoters reporter plasmid
Plasmid#156457PurposeA mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.DepositorInsertNeoR/KanR
ExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7Available SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)
Plasmid#156463PurposeExpress Heme Oxygenase (Nostocaceae) and ReBphP-PCMDepositorInsertsHeme Oxygenase
ReBphP-PCM
TagsHis TagExpressionBacterialPromoterT7Available SinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)
Plasmid#159697PurposeE. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)
Plasmid#159579PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)DepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mCherry
Plasmid#145279PurposeBacterial Expression of 6xHis mCherry Fusion ProteinDepositorInsertmCherry
ExpressionBacterialAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-AK74
Plasmid#159594PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at AK74
ExpressionBacterialAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL5-pCMV7.1
Plasmid#155024PurposeN-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vectorDepositorAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-GFP-C-LACE
Plasmid#133913PurposeBacterial expression of His6-GFP-C-LACEDepositorInsertsuperfolder green fluorescent protein
TagsLACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…ExpressionBacterialPromoterT7Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pADsacA
Plasmid#158649PurposeConstitutive transcription of sacA crRNA and GFPDepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…ExpressionBacterialPromoterPvegAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT03-Apr
Plasmid#154756PurposeFor curing of the resistance marker of RecET recombinase system in Acinetobacter baumannii, AprarDepositorInsertFLP recombinase
ExpressionBacterialAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
P22_wtCP_SP141his
Plasmid#122651PurposeExpression of P22 virus like particle in bacteriaDepositorInsertP22 Coat: WT / SP: 6xHis-SP141
ExpressionBacterialMutationtruncated SP, P120H in the capsid scaffold (see …Available SinceAug. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDonor-GDH
Plasmid#140629PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.DepositorInsertMini-Tn6677, T7lac-GDH gene cassette
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF_LuxR
Plasmid#140039PurposeConstutitive expression of LuxR protein.DepositorInsertpLuxL and LuxR
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mNeonGreen-EC18153
Plasmid#137075PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136458
Plasmid#149531PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046754
Plasmid#145647PurposeBacterial Expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046814
Plasmid#145601PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p_SNIPR_sensor_GFP
Plasmid#139463PurposeT7 RNAP-driven expression of SNIPR RNA with a GFP-ASV reporterDepositorInsertSNIPR_GFP
ExpressionBacterialAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Citroff1
Plasmid#134301PurposeBacterial expression of inverse-response citrate sensor with high binding affinityDepositorInsertCitroff1
Tags6-His tagExpressionBacterialPromoterpBADAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only