33,449 results

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pET29b_1_anti-GFP-nanobody

Plasmid

#210394

Purpose

Express anti-GFP nanobody in E. coli

Depositor

Magnus Rueping

Publication

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

1_anti-GFP-nanobody

Tags

6xHis

Expression

Bacterial

Available since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b-ULP1-405-621

Plasmid

#229224

Purpose

For expression of ULP1 protein

Depositor

Andy LiWang

Insert

ULP1 (ULP1 Budding Yeast)

Expression

Bacterial

Mutation

405-621 (truncated)

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET24a isPETase

Plasmid

#225759

Purpose

Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

Depositor

Jason Boock

Publication

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

isPETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AncMEcoGII291

Plasmid

#205024

Purpose

Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

Depositor

Paola Laurino

Publication

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

Ancestral M.EcoGII 291

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher

Plasmid

#223633

Purpose

Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His6

Expression

Bacterial

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Butelase AY

Plasmid

#221847

Purpose

Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

Depositor

Artur Krężel

Publication

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

Butelase 1 ligase

Expression

Bacterial

Mutation

Val237Ala, Thr238Tyr

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-EF-Ts-His6

Plasmid

#224371

Purpose

Express EF-Ts protein for his-tagged purification

Depositor

Richard Murray

Publication

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

EF-Ts (tsf E. coli)

Tags

His6

Expression

Bacterial

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-EF-G-His8

Plasmid

#224369

Purpose

Express EF-G protein for his-tagged purification

Depositor

Richard Murray

Publication

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

EF-G (fusA E. coli)

Tags

His8

Expression

Bacterial

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-PheRSa-PheRSb

Plasmid

#224364

Purpose

Express PheRS protein for his-tagged purification

Depositor

Richard Murray

Publication

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

PheRS (pheS E. coli)

Tags

His6

Expression

Bacterial

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pNP2270

Plasmid

#222733

Purpose

pNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase

Depositor

Patrick Hsu

Publication

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 Helper (lacZ)

Expression

Bacterial

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-DepoPETase

Plasmid

#218702

Purpose

Encodes codon optimized DepoPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

DepoPETase

Tags

10xHis

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-RSET-iGABASnFR2-6xHis

Plasmid

#218885

Purpose

Bacterial expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Publication

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2

Tags

6xHis

Expression

Bacterial

Mutation

S99A F102Y F104Y L178S

Promoter

T7

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptac

Plasmid

#218357

Purpose

Empty vector with lacIq-Ptac expression system

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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mTB3-BC2 nanobody

Plasmid

#210208

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

Depositor

Brian Kay

Publication

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

BC2 Nanobody

Expression

Bacterial

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-ER-MT-P2A

Plasmid

#214892

Purpose

Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

Depositor

Tito Calì

Insert

SPLICSFAST-ER-MT-P2A

Tags

none

Expression

Bacterial and Mammalian

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pETM43 p62 FL (1-440)

Plasmid

#210425

Purpose

Bacterial expression of MBP-p62 FL (1-440) with 3C cleavage site

Depositor

Carsten Sachse

Publication

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 FL (NUP62 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attR4-pegRNA-attR3

Plasmid

#213056

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attL4

Plasmid

#213055

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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OmpF

Plasmid

#213135

Purpose

Expresses outer membrane protein F

Depositor

Sebastian Hiller

Insert

OmpF (23-362) (ompF E.coli (K12))

Expression

Bacterial

Promoter

T7

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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