Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,417 results

Showing: 6021 - 6040 of 32417 results

  3. pCJR01

    Plasmid
    #187888
    Purpose
    Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.
    Depositor
    Gaylen Uhlich
    Article
    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144
    Insert
    DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187888-plasmid-map-sequence-id-368412

    Enlarge

  5. pBFC1207

    Plasmid
    #186694
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186694-plasmid-map-sequence-id-371385

    Enlarge

  6. TRMT10C (RG9MTD1A-c113)

    Plasmid
    #110248
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RG9MTD1 (TRMT10C Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110248-plasmid-map-sequence-id-212286

    Enlarge

  8. pFnCpf1GG

    Plasmid
    #131010
    Purpose
    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of FnCas12a
    GFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131010-plasmid-map-sequence-id-257701

    Enlarge

  9. KDM4D

    Plasmid
    #73247
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KDM4D (KDM4D Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam02375:JmjN 18-64, pfam02373:JmjC 182-295
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73247-plasmid-map-sequence-id-137202

    Enlarge

  13. AK3L1

    Plasmid
    #39135
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AK3L1 (AK3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pAL-rfp_T

    Plasmid
    #74075
    Purpose
    pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA rfp (T)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available Since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74075-plasmid-map-sequence-id-141966

    Enlarge

Showing: 6021 - 6040 of 32417 results