32,453 results

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pET3aTr

Plasmid

#64008

Purpose

Single donor or transfer plasmid for source of translation cassette for plasmid suite

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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p6187

Plasmid

#198158

Purpose

bacterial expression of sifA::HaloTag fusion, mutant form

Depositor

Michael Hensel

Insert

PsifA sifA delta331-336::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)

Tags

HaloTag-HA

Expression

Bacterial

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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CJ8421_04975 Rptr2 and CJ8421_04975 mRNA

Plasmid

#194210

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and CJ8421_04975 Rptr2

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET GFP domain 11 LIC cloning vector (GFP C-11)

Plasmid

#30183

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), GFP domain 11, and TEV cl…

Expression

Bacterial

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pJF143.J3

Plasmid

#153028

Purpose

J3-mRFP1

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

J3-mRFP1

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_FL

Plasmid

#187712

Purpose

Express GST-tagged NEDD4L FL in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV109

Plasmid

#215339

Purpose

Expresses sfGFP under induction with the 3OC14-HSL Marionette Cassette (CinRAM + PCin-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PCin

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJ2076.2_TS

Plasmid

#140667

Purpose

Toggle switch (TS)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Toggle switch (TS)

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pETDuet-TFIIE

Plasmid

#171083

Purpose

Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Inserts

TF2E1 (GTF2E1 Human)
TF2E2 (GTF2E2 Human)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT17

Plasmid

#89660

Purpose

expression of doubleTAL staple protein dT17 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT17

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-mYFP-hphMX6

Plasmid

#105155

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-hphMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

Plasmid

#182424

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
VNp-LZ

Tags

Vesicle Nucleating peptide (VNp), and Vesicle Nuc…

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPro33

Plasmid

#17810

Depositor

Jay Keasling

Article

Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

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pdCas9+sgRNA expression vector precursor

Plasmid

#171798

Purpose

PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

Depositor

Stephen Elledge

Article

Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO

Available Since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMM627

Plasmid

#112532

Purpose

pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA
luxCDABE

Expression

Bacterial

Promoter

J23107, PL(HrtO), and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-Opt13

Plasmid

#61698

Purpose

Expresses PTDH mutant Opt13

Depositor

Huimin Zhao

Article

McLachlan et al Biotechnol Bioeng. 2008 Feb 1;99(2):268-

Insert

Opt13

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2015

Availability

Academic Institutions and Nonprofits only
Reciever_plasmid2

Plasmid

#140695

Purpose

Encodes quorum sensing (C4-HSL) inducible cyan (sfCFP) expression

Depositor

Matthew Bennett

Article

Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

Insert

sfCFP

Expression

Bacterial

Promoter

engineered rhl promoter (responds to C4-HSL)

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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GEV 2

Plasmid

#12616

Depositor

John Louis

Article

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)

Tags

G protein and His

Expression

Bacterial

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pAB152

Plasmid

#101663

Purpose

Opto-T7RNAP*(563-F2)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-B*07:02-BirA

Plasmid

#239752

Purpose

E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-B*07:02 (HLA-B Human)

Tags

BirA substrate peptide

Expression

Bacterial

Mutation

G120C

Promoter

T7

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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