32,947 results

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PET-WT SpCas9-NLS-6xHis

Plasmid

#207373

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

WT SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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plYJP022-int7

Plasmid

#202287

Purpose

Plasmid containing integrase for LPattB7 site

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

int7

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-TorA(sp)-mNeonGreen

Plasmid

#205020

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorA-mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

Pc

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pPath-Violacein-SGE

Plasmid

#200003

Purpose

Violacein pathway. SGE

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET47b-T7-AsCas12f-WT

Plasmid

#204640

Purpose

Expression of wild-type AsCas12f in E. coli

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

AsCas12f

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-MED1LCD

Plasmid

#204404

Purpose

E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

MED1(947-1581) (MED1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-MCS-NLS-3XFLAG-V5

Plasmid

#196383

Purpose

Empty backbone vector to insert cDNA for strong expression in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Type

Empty backbone

Tags

3xFLAG and V5

Expression

Bacterial and Mammalian

Promoter

CAGGs

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-LIC-N8C_Fab3b

Plasmid

#203685

Purpose

Expresses N8C_Fab3b

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab3b

Expression

Bacterial

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET30(a)-AtNUDX23

Plasmid

#175021

Purpose

Purification of AtNUDX23 enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

AtNUDX23 (NUDX23 Mustard Weed)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH4-6

Plasmid

#199766

Purpose

Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH46

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/pT7 LasI

Plasmid

#193631

Purpose

Constitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.

Depositor

Dora Tang

Article

Gonzales et al ChemSystemsChem 2023

Insert

LasI

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNAP promoter

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCS-lpp0.2-K64A

Plasmid

#201789

Purpose

mutant of PadR; transcriptional regulator can respond to p-coumaric acid

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PadR (padR Bacillus subtilis)

Expression

Bacterial

Mutation

K64A

Available Since

July 12, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET24b-BG1.3

Plasmid

#199437

Purpose

expresses BAmGreen1.3 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMGreen1.3

Tags

his tag

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-dCas12m

Plasmid

#192275

Purpose

Encodes MmdCas12m (D485A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m

Use

CRISPR

Expression

Bacterial

Mutation

D485A

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTarget: Ptaq-RFP_PlacIq-GFP

Plasmid

#192280

Purpose

RFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter Placiq

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Inserts

RFP
GFP

Expression

Bacterial

Promoter

PlacIq and Ptaq

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-pML-1B_6xHis-Cas12m

Plasmid

#192282

Purpose

MmCas12m fused to His-tag for protein purification

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12m

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-FNLDTA

Plasmid

#195340

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis Tag and MBP, TEV cleavage site, 6x His Tag

Expression

Bacterial

Mutation

F208W, N524V, L795I, D868V, T874S, A1015E

Promoter

T7 Promoter

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBZS-M.CviPII (NDel15)

Plasmid

#198356

Purpose

Express M.CviPII (NDel15) in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

M.CviPII

Expression

Bacterial

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMUT2-pCadBA-mCherry

Plasmid

#192865

Purpose

pMUT2 based vector for sensing pH in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

pH sensitive promoter

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Ezr-BioID2-HA

Plasmid

#176744

Purpose

Expresses Ezrin fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Ezr-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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