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pAOJ15

Plasmid

#91567

Purpose

Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter

Depositor

Harry Mobley

Article

Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

Type

Empty backbone

Expression

Bacterial

Promoter

Tetracycline promoter

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMS12: pDonor(AvCAST)

Plasmid

#168145

Purpose

AvCAST donor harboring non-functional cargo.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST right end (RE)
AvCAST left end (LE)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pV1F(Spec)

Plasmid

#205086

Purpose

Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

Depositor

Suomeng Dong

Article

Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

Insert

AtVIP1

Expression

Bacterial

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1A-SynTetOff-GFP

Plasmid

#190230

Purpose

Gateway entry vector, high-level expression of EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Expression

Bacterial

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCJ208

Plasmid

#162681

Purpose

Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.

Depositor

Gregg Beckham

Article

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Insert

PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid

Tags

His

Expression

Bacterial

Mutation

Replacement of W185-F191 with G251-T472 from MHET…

Promoter

T7/lac

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEC3079 (p7INT-Pveg_lacI)

Plasmid

#218505

Purpose

integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

lacI (lacI Escherichia coli)

Expression

Bacterial

Promoter

Pveg

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMesh1

Plasmid

#175594

Purpose

Lac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

Depositor

Greg Bokinsky

Article

Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

Insert

Mesh1 (Mesh1 Fly)

Expression

Bacterial

Mutation

Codon optimized for translation in E.coli. AGGAGG…

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IGF2BP3-Halo

Plasmid

#175310

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP3 (IGF2BP3 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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BPTF PHD and Bromodomain

Plasmid

#92101

Purpose

Expresses GST fusion PHD and Bromodomain from BPTF protein

Depositor

Albert Jeltsch

Article

Mauser et al Biochimie. 2018 Mar;146:14-19. doi: 10.1

Insert

PHD and Bromodomain (BPTF Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_PABPC1_PABP

Plasmid

#109931

Purpose

Protein expression and purification of PABPC1_PABP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PABPC1_PABP

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET26b-SET

Plasmid

#8822

Depositor

Judy Lieberman

Article

Beresford et al J Biol Chem 2001 Nov 16;276(46):43285-93

Insert

SET (SET Human)

Tags

6XHis

Expression

Bacterial

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
p007ampGFP

Plasmid

#58535

Purpose

GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

Green fluorescent protein

Expression

Bacterial

Promoter

T5 between two lac operators

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET co-transformation cloning vector (13S-A)

Plasmid

#48323

Depositor

Scott Gradia

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pGI3EM22C

Plasmid

#135488

Purpose

Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr LifeAct-tdTomato

Tags

LifeAct

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ScSpe1 pET-20b(+)

Plasmid

#117145

Purpose

Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

Depositor

Markus Ralser

Article

Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

Insert

SPE1

Tags

Histidine

Expression

Bacterial

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HSPA5-Halo

Plasmid

#175326

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA5 (HSPA5 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-SPA2G231V

Plasmid

#42079

Depositor

Christine Garcia

Article

Maitra et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

Surfactant Protein -A2 (SFTPA2 Human)

Use

Lentiviral; Plenti

Tags

V5

Expression

Bacterial and Mammalian

Mutation

Changed Glycine 231 to Valine

Promoter

CMV

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
(SUMO)5

Plasmid

#126949

Purpose

Bacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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p42.3A7

Plasmid

#69575

Purpose

OT-I TCR a chain cDNA in pCR1000

Depositor

Francis R. Carbone

Article

Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

Insert

TCR alpha clone 149.42 (Tcra Mouse)

Expression

Bacterial

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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