34,239 results
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Plasmid#232211PurposeGGA cloning MSG-AGGA-promoter-ccdb-TTCC-HISDepositorInsertccdb
ExpressionBacterialPromoterT7Available sinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis
Plasmid#246982PurposeExpresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.DepositorInsertφC31(PhiC31)
UseSynthetic BiologyTags8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrepExpressionBacterialPromoterT7Available sinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HER2Nb-C AND (A OR T)-SpyTag
Plasmid#243790PurposeExpresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.DepositorInsertHER2 nanobody
Tags6x His tag and SsrAExpressionBacterialAvailable sinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNoRBP.iGlucoSnFR2
Plasmid#244104PurposeBacterial expression of green glucose sensorDepositorInsertiGlucoSnFR2
ExpressionBacterialAvailable sinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
TwinStrepSUMO-pvc10-Cas9
Plasmid#243755PurposeFor affinity purification of Pvc10 fused to Cas9DepositorInsertpvc10-Cas9
ExpressionBacterialAvailable sinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5_HUHe-Pvc10-Cas9
Plasmid#243744PurposePVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on NTD and Cas9 on CTDDepositorInsertpvc13-Ad5_HUHe-Pvc10-Cas9
ExpressionBacterialAvailable sinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEVA621-AzFRS
Plasmid#242002PurposeExpressed AzF tRNA synthetase for incorporation of p-azido-L-phenylalanine (pAzF)DepositorInsertAzFRS
ExpressionBacterialAvailable sinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS
Plasmid#244833PurposeBacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purificationDepositorInsertSpyCas9 (WT)
UseCRISPRTags2xSV40 NLS and 10xHis-MBPExpressionBacterialPromoterT7Available sinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28(a)-p2c-Cas9
Plasmid#242759PurposeExpresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coliDepositorInsertP2C-Cas9
UseCRISPRExpressionBacterialPromoterlacAvailable sinceSept. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pREC1010-Kva1
Plasmid#240684PurposeRSF1010 origin of replication plasmid containing Kva1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoterDepositorInsertKva1 RT,Kva1 ncRNA, Beta and EcSSB
ExpressionBacterialMutationWTAvailable sinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF1-Pls1-sfGFP
Plasmid#205748PurposeExpresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.DepositorTypeEmpty backboneUseShuttle vector gram+ gram-ExpressionBacterialPromoterPls1 (Phyperspank mutant, IPTG inducible)Available sinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28_Cas9d_Plasmid
Plasmid#239445PurposeExpression plasmid codon-optimized for E. coliDepositorInsertCas9d
ExpressionBacterialPromoterT7Available sinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-GST-Tev-Af1521(K35E/Y145R)-myc
Plasmid#196241PurposeN-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminusDepositorInsertN-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
TagsGST tag and myc tagExpressionBacterialMutationamino acid 35 lysine is replaced with glutamic ac…Available sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET30a-GABAT
Plasmid#239572PurposeExpresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.DepositorAvailable sinceJuly 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSJX022
Plasmid#232325PurposeGenome editing in Caulobacter crescentusDepositorInsertssgRNA
SpCas9M
UseCRISPRTagsH-arms and catExpressionBacterialPromoterPJ23119 and PvanAvailable sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSIG1
Plasmid#226908PurposeMoClo-compatible vectors for dsRNA production in E. coliDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7 promoterAvailable sinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBTK1126
Plasmid#236187PurposeBsaI compatible gentamicin resistance cassetteDepositorInsertgentamicin resistance cassette
ExpressionBacterialPromoterCP25Available sinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ14112 tyrosinase-targeting sgRNA
Plasmid#239751Purposeguide for tyrosinaseDepositorInsertguide targeting tyrosinase
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ14103 aeBlue-targeting_sgRNA
Plasmid#239750Purposeguide for aeBlueDepositorInsertguide targeting aeBlue
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
p300dAIL KAT LIC 1M
Plasmid#233588PurposeBacterial expression of p300 KAT enzyme with truncated autoinhibitory loopDepositorInsertp300dAIL KAT (EP300 Human)
Tags6xHis MBPExpressionBacterialMutationdeleted amino acids 1523-1554PromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by