33,532 results
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Plasmid#243750PurposeFor affinity purification of spike tip (Pvc10)DepositorInsertpvc10
ExpressionBacterialAvailable sinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
TwinStrepSUMO-Ad5-SpyCatcher
Plasmid#243751PurposeFor affinity purification of A5 knob fused to SpyCatcherDepositorInsertAd5-SpyCatcher
ExpressionBacterialAvailable sinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBR322-sgRNA-pvc18-20-Pdp1_NTD-Cre
Plasmid#243748PurposePVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA - Cre loaded into the PVC tube via Pdp1 (NTD)DepositorInsertvegfa sgRNA-pvc18-20-Pdp1_NTD-Cre
ExpressionBacterialAvailable sinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5_pvc10-HUHe
Plasmid#243745PurposePVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on CTDDepositorInsertpvc13-Ad5_pvc10-HUHe
ExpressionBacterialAvailable sinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEVA621-AzFRS
Plasmid#242002PurposeExpressed AzF tRNA synthetase for incorporation of p-azido-L-phenylalanine (pAzF)DepositorInsertAzFRS
ExpressionBacterialAvailable sinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS
Plasmid#244833PurposeBacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purificationDepositorInsertSpyCas9 (WT)
UseCRISPRTags10xHis-MBP and 2xSV40 NLSExpressionBacterialPromoterT7Available sinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKOS6b-KOpstS
Plasmid#237503PurposeKnockout of pstSDepositorInsertphosphate ABC transporter substrate-binding protein PstS
ExpressionBacterialAvailable sinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKOS6b-URP112
Plasmid#237504PurposeUpregulation of mgdh with P112DepositorInsertmembrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
ExpressionBacterialAvailable sinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28(a)-p2c-eGFP
Plasmid#242758PurposeExpresses eGFP fluorophore with fused p2c targeting ligand for expression in E. coliDepositorInsertP2C-EGFP
ExpressionBacterialAvailable sinceSept. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRECBRR1-Kva1
Plasmid#240703Purposeinverted BRR1 origin of replication plasmid containing Kva1 recombitron with a donor in the ncRNA to target phzM locus in Pseudomonas aeruginosa PAO1 expressed by Pm promoterDepositorInsertKva1 RT,Kva1 ncRNA, PaRecT and PaSSB
ExpressionBacterialMutationInverted BRR1 origin of replicationAvailable sinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSSBIO32_dCas9_RPA1786
Plasmid#240489PurposeCRISPRi-mediated Knockdown of RPA1786DepositorInsertCas9 (cas9 Streptococcus pyogenes)
UseCRISPRExpressionBacterialMutationD10A, H840APromoterPlacAvailable sinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-GST-Tev-Af1521-myc
Plasmid#196239PurposeN-terminal GST fusion of Af1521(WT) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminusDepositorInsertN-terminal GST fusion of Af1521(WT) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
TagsGST tag and myc tagExpressionBacterialAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-GST-Tev-Af1521(K35E/Y145R)-myc
Plasmid#196241PurposeN-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminusDepositorInsertN-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
TagsGST tag and myc tagExpressionBacterialMutationamino acid 35 lysine is replaced with glutamic ac…Available sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-ALFA-TwinStrep
Plasmid#240248PurposeArabinose-inducible plasmid with ALFA and Twin-Strep tagsDepositorPublicationTypeEmpty backboneTags10x HIS, ALFA, Thrombin, and Twin-StrepExpressionBacterialPromoteraraBADAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pFUSE-rIgG-Fc2-MFSD8(L9)-Zeo
Plasmid#239947PurposeExpression of MFSD8 loop 9 tagged with rabbit IgG Fc2DepositorInsertFc2-MFSD8(L9)
ExpressionBacterial and MammalianAvailable sinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas12a-NT
Plasmid#235167PurposeExpresses the Wild Type Francisella novicida cas12a gene and a NT crRNADepositorInsertFnCas12a
ExpressionBacterialAvailable sinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
TDP43-NTD
Plasmid#241024PurposeBacterial expression of TDP-43 NTDDepositorInsertTDP43-NTD
Tags6x HisExpressionBacterialMutationchanged N81 to stop codonAvailable sinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS2096
Plasmid#239835PurposeNovaIscB E. coli expression for recombinant purificationDepositorInsertNovaIscB
Tags14xHis-TwinStrep-bdSUMOExpressionBacterialMutationInsertion of REC domain from NbaCas9-1 and swap o…PromoterT7Available sinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET30a-GABAT
Plasmid#239572PurposeExpresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.DepositorAvailable sinceJuly 18, 2025AvailabilityAcademic Institutions and Nonprofits only