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PTPRE

Plasmid

#38950

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PTPRE (PTPRE Human)

Tags

His6

Expression

Bacterial

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2_N_deltaNTD

Plasmid

#157869

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domain

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaNTD

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pPPC011

Plasmid

#171143

Purpose

Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Sp.pCas9, BBa_J23107

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-LSSmCherry1(-13)

Plasmid

#219415

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

LSSmCherry1(-13)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

K137E, K199E

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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mTB3-Myc tag

Plasmid

#210205

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

Myc-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMB2a

Plasmid

#190120

Purpose

Level 2 MoClo plasmid for A. baumannii

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-29

Plasmid

#172753

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-29

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-cxcr4b whim-gfp

Plasmid

#41089

Depositor

Anna Huttenlocher

Article

Walters et al Blood. 2010 Oct 14;116(15):2803-11. Epub

Insert

CXCR4b-WHIM (cxcr4b Zebrafish)

Tags

GFP

Expression

Bacterial

Mutation

deleted last 19 amino acids of cxcr4b

Promoter

mpx

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pTYB2-eIF1

Plasmid

#37217

Depositor

Jon Lorsch

Article

Acker et al Methods Enzymol. 2007;430:111-45.

Insert

eIF1

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pHH0103_BCL2L1_BH4+Bcl-2

Plasmid

#109993

Purpose

Protein expression and purification of BCL2L1_BH4+Bcl-2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

BCL2L1_BH4+Bcl-2 (BCL2L1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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env8 selectivity (b')

Plasmid

#99837

Purpose

mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7

Insert

env8 selectivity (b')

Tags

mNeonGreen

Expression

Bacterial

Mutation

A20G, A68G, G-C pairs in P1 and P3

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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4Z3C (TET3)

Plasmid

#119969

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

TET3 (TET3 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

contains amino acids 48-98

Promoter

T7

Available Since

Jan. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCool-hDDB1-BPB

Plasmid

#48639

Purpose

Expresses DDB1 BPB domain (aa 391-709) in Ecoli (ampR)

Depositor

Ning Zheng

Article

Li et al Cell. 2006 Jan 13;124(1):105-17.

Insert

hDDB1-BPB (DDB1 Human)

Expression

Bacterial

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only
CoV-2-NSP3-c001

Plasmid

#173083

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))

Tags

mCerulean

Expression

Bacterial

Mutation

E1084-E1192, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx4-H6-FGB

Plasmid

#49203

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

H6-FGB

Tags

His tag + Other tags

Expression

Bacterial, Insect, and Mamm…

Mutation

None

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pDM098

Plasmid

#216818

Purpose

Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mStayGold

Tags

FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDUP1

Plasmid

#223516

Purpose

inactive cogfp under control of Ptet, cogfp under control of Ptac

Depositor

Yolanda Schaerli

Article

Mihajlovic et al eLife. 2025 Apr 2;13:RP97216. doi: 10.75

Inserts

CoGFP
CoGFP inactivated

Tags

His tag

Expression

Bacterial

Mutation

anti-dimerization mutations S129G, 22 C154S, D156…

Promoter

Ptac, Ptac and Ptet, and Ptet

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBad-bapaUGAc

Plasmid

#201673

Purpose

boronic acid- and peptide-assisted UDP-GlcNAc sensor

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

Insert

bapaUGAc

Tags

Hisx6

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_RAB5A_Ras

Plasmid

#109935

Purpose

Protein expression and purification of RAB5A_Ras

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAB5A_Ras

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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As2

Plasmid

#123160

Purpose

Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR-ABS84, PhrpLE

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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