34,239 results

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pBbB14a-GFP

Plasmid

#126117

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Publication

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pC-0_v6

Plasmid

#124426

Purpose

Full MCS, lacks araC & P(BAD) (step 2)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pC-0

Plasmid

#124421

Purpose

Full MCS (step 2)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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His6_BcLOV4_Q355N_mCherry_BamUK

Plasmid

#119762

Purpose

Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

Depositor

Brian Chow

Publication

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-Q355N_mCherry

Tags

mCherry

Expression

Bacterial

Mutation

Mutated glutamine at position 355 to asparagine

Promoter

T7

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163 QQ4xSS #1

Plasmid

#127194

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Publication

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #1 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET_RFP-SZ2

Plasmid

#124933

Purpose

Expression of RFP-SZ2

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-SYNZIP2

Tags

His and mCherry

Expression

Bacterial

Mutation

SZ2 domain

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW1658

Plasmid

#119179

Purpose

E.coli expression plasmid for Pm4CL1 and PmSPS2

Depositor

Jing-Ke Weng

Publication

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Inserts

Pm4CL1
PmSPS2

Expression

Bacterial

Promoter

pGAP

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW0770

Plasmid

#119023

Purpose

E.coli expression plasmid for PmSPS1

Depositor

Jing-Ke Weng

Publication

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmSPS1

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RGG_withTEVsite

Plasmid

#124940

Purpose

Expression of RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Mutation

with TEV site between RGG domains

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-848-952(pL91)

Plasmid

#124388

Purpose

E.Coli produced TET1-848-952 purified by FPLC nickel column

Depositor

Shaun Cowley

Publication

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-848-952 wildtype (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV20-1

Plasmid

#123454

Purpose

construction of TCR

Depositor

Catherine Wu

Publication

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV20-1 (TRBV20-1 Human)

Expression

Bacterial

Mutation

WT

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV6-5

Plasmid

#123425

Purpose

construction of TCR

Depositor

Catherine Wu

Publication

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV6-5 (TRBV6-5 Human)

Expression

Bacterial

Mutation

WT

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSELt_S7.A

Plasmid

#125835

Purpose

Negative selection plasmid for evolution of an amber stop codon suppression tRNA

Depositor

Andrew Ellington

Publication

Maranhao et al ACS Synth Biol. 2017 Jan 20;6(1):108-119

Insert

ACP-2xAmb-pheS

Use

Synthetic Biology

Expression

Bacterial

Promoter

pr.trpL*

Available since

May 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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TRBV5-1

Plasmid

#123414

Purpose

construction of TCR

Depositor

Catherine Wu

Publication

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV5-1 (TRBV5-1 Human)

Expression

Bacterial

Mutation

WT

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pXWJ109AsAmp30E

Plasmid

#123144

Purpose

Arsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Publication

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR, PhrpLE

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pXWJ109HgAmp30E

Plasmid

#123149

Purpose

Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Publication

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, PmerT, PhrpLE

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pXWP11-gfp2

Plasmid

#123152

Purpose

pSB1K3 carrying Pecf11-30gfp-t

Depositor

Baojun Wang

Publication

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

Pecf11-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf11

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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mNG-mTq2

Plasmid

#124217

Purpose

Cytoplasmic mNeongreen mTurquoise2 tandem, ~60 % energy transfer. pNM043 - pSAV057-mNG-mTq2

Depositor

Tanneke den Blaauwen

Publication

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

mNG-mTq2

Expression

Bacterial

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-mRFP1 gRNA

Plasmid

#117052

Purpose

In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

Depositor

Michael Jewett

Publication

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

anti-mRFP1 SpCas9 gRNA

Expression

Bacterial

Promoter

T7

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TRAV26-2

Plasmid

#123399

Purpose

construction of TCR

Depositor

Catherine Wu

Publication

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV26-2 (TRAV26-2 Human)

Expression

Bacterial

Mutation

WT

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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