33,539 results
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Plasmid#232824PurposeRandom transposon mutagenesis vector for Bacteroidales species - erythromycinDepositorInsertermG
ExpressionBacterialAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH81 pET21b Rat1-His
Plasmid#231650PurposeBacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tagDepositorAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH82 pET28a-Rai1
Plasmid#231651PurposeBacterial expression vector expressing yeast S. cerevisiae rai1, no tagDepositorInsertrai1 (RAI1 Budding Yeast)
ExpressionBacterialAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF-RFP amber
Plasmid#229524PurposemCherry red fluorescent protein (RFP) reporter protein with an amber stop codon at position 13DepositorPublicationInsertRFP amber
ExpressionBacterialPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmNG
Plasmid#232200PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
ExpressionBacterialAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
WNV isolate WN NY 2000-crow3356 NS5 RdRp full length
Plasmid#228662PurposeBacterial expression of the West Nile virus NS5 RdRp protein for biochemical assayDepositorInsertWest nile NS5 RdRp
TagsHis-SUMOExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus D68 (EV-D68) strain EV-D68 STL 2014 12 3C protease
Plasmid#228644PurposeBacterial expression of the D68 3C proteaseDepositorInsertEnterovirus D68 3C protease
TagsHis-SUMOExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease
Plasmid#228637PurposeBacterial expression of the Coxsackievirus 16 3C proteaseDepositorInsertCoxsackievirus 16 3C protease
TagsHis-SUMOExpressionBacterialAvailable sinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease
Plasmid#228636PurposeBacterial expression of the A71 3C protease (isoform B) used in crystallisationDepositorInsertEnterovirus A71 3C protease isoform B
TagsHis-SUMOExpressionBacterialAvailable sinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMVRha
Plasmid#229553PurposeBroad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-SisTnpB1–ωRNA
Plasmid#228365PurposeExpresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cellsDepositorInsertSisTnpB1
ExpressionBacterialPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-321_Cacnes_ori
Plasmid#225603PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-400_Cacnes_CAMP2
Plasmid#225606PurposeSuicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassetteDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-224_Cacnes_sfGFP
Plasmid#225611PurposeReplicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.DepositorInsertsfGFP
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-164_Cacnes_pFAST
Plasmid#225616PurposeReplicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.DepositorInsertpFAST
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1mTEAD
Plasmid#230916PurposeComparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.DepositorInsertHuman COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
UseLuciferaseTagsLuciferase- luc2pExpressionBacterial and MammalianMutationmutant potential TEAD bind elements AGGAAT to CTG…PromoterCOL1A1Available sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+) pCopA RBS30 mscarlet
Plasmid#226370PurposePlasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30DepositorInsertmScarlet
Tags6x HistagExpressionBacterialPromotercopA promoter (pCopA)Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry_YafQ/DinJ
Plasmid#229148PurposeToxin-Antitoxin (Dinj/YafQ)DepositorInsertmCherry, DinJ/YafQ
ExpressionBacterialPromoterPtlpA (mCherry), DinJ/YafQ (Native)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-MjCouRS
Plasmid#229066PurposetRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codonDepositorInsertMethanococcus jannaschii tRNA synthetase
ExpressionBacterialPromoterT7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSB19-PapRS#34-OmpA
Plasmid#231105PurposeVector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)DepositorTypeEmpty backboneExpressionBacterialAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only