34,239 results
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Plasmid#239055PurposeBacterial expression of high affinity red fluorescent pyruvate biosensor ApplePy1HighDepositorPublicationInsertApplePy1High
ExpressionBacterialAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD-dApplePy1
Plasmid#239058PurposeBacterial expression of red fluorescent pyruvate control biosensor dApplePy1DepositorPublicationInsertdApplePy1
ExpressionBacterialAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD-GreenPy1Lowest
Plasmid#239052PurposeBacterial expression of lowest affinity green fluorescent pyruvate biosensor GreenPy1LowestDepositorPublicationInsertGreenPy1Lowest
ExpressionBacterialAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSJX024
Plasmid#232327PurposeGenome editing in Caulobacter crescentusDepositorInsertssgRNA
SpCas9M
UseCRISPRTagsH-arms and catExpressionBacterialPromoterPJ23119 and PxylAvailable sinceJune 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc RFC[2+3+4]-pET
Plasmid#239203PurposeOverexpress Sc RFC2, RFC3, & RFC4 in E. coliDepositorExpressionBacterialAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLS410
Plasmid#239123PurposeMinimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMXDepositorTypeEmpty backboneUseCentromericExpressionBacterial and YeastAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
ptet-lux-Tn7T-Gm
Plasmid#226434PurposeThis vector features a tetracycline-inducible system to regulate luxABCDE reporter expression, optimized for efficiency in P. aeruginosa.DepositorInsertsoptimized TetR
luxABCDE
ExpressionBacterialPromoterPcon and PtetAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-28c-F30-2xdBroccoli-47CAG
Plasmid#237217PurposeExpress F30-2xdBroccoli tagged 47 repeats of CAG RNA in bacteria.DepositorInsert47 CAG trinucleotide repeats RNA
ExpressionBacterialAvailable sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-30a-NSP14-SARS-CoV-2
Plasmid#236423Purposebacterial protein expression of NSP14DepositorAvailable sinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
PrecA-CFP--PrecA-YFP
Plasmid#228097PurposerecA promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporterDepositorInsertCFP & YFP
ExpressionBacterialPromoterrecA & recAAvailable sinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-T4gp43(WT)
Plasmid#228203PurposeExpression in BL21(DE3) of WT T4 DNA polymeraseDepositorInsert43
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-RoTq-On
Plasmid#234465PurposeBacterial expression vector for RoTq-OnDepositorInsertRoTq-On
Tags7xHisExpressionBacterialAvailable sinceMay 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLuc-N-HiBiT
Plasmid#207117PurposeBacterially expressed HiBiT-FLuc for Ni-NTA purificationDepositorInsertluciferin 4-monooxygenase
TagsHiBiT Tag and His-tag with thrombin siteExpressionBacterialMutationCodon optmization for expression in BL21DE3PromoterT7 PromotorAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK316
Plasmid#235474PurposeInducible gene VIII by Ptet promoterDepositorInsertphage M13 geneVIII
ExpressionBacterialAvailable sinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK346
Plasmid#235477PurposeInducible gene VIII by Pbad promoterDepositorInsertphage M13 geneVIII
ExpressionBacterialAvailable sinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG054
Plasmid#231323PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmRDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMW364
Plasmid#238205PurposeExpresses untagged TasR from a PBAD promoter, followed by 3xRNA1 TIGR arrayDepositorInsertTaTasR
ExpressionBacterialAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG_UTu1D
Plasmid#233867PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistantDepositorInsertsallo-tRNA UTu1D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
ExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG) and …PromoterEM7, araBAD, and native As SelDAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
BLA_CAT_K136_GPG_LOV2
Plasmid#231575PurposeThis plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue lightDepositorInsertCAT_ carrying an AsLOV2 insertion behind K136
ExpressionBacterialAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTn7xTS_SpyCatcher:mCitrine
Plasmid#225187PurposeUsed for inserting SpyCatcher fused to mCitrine at the bacterial Tn7 siteDepositorInsertSpyCatcher:mCitrine
TagsSpyCatcherExpressionBacterialAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only