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33,539 results

Showing: 11701 - 11720 of 33539 results

  1. pQ-Elo-HB-C-A(1-772)

    Plasmid
    #171085
    Purpose
    Co-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Publication
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26 amino acids for expression purp…
    Promoter
    T5 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171085-plasmid-map-sequence-id-335501

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  7. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid
    #222099
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Publication
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Vitiosangium-bGSDM-thrombin ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222099-plasmid-map-sequence-id-437094

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  10. pBV514

    Plasmid
    #204602
    Purpose
    Expresses F74A OsTir1-3X Myc from S. cerevisiae ADH1 promoter
    Depositor
    Scott Moye-Rowley
    Insert
    Os Tir1 (LOC4335696 Oryza sativa)
    Tags
    Myc epitope tags
    Expression
    Bacterial
    Mutation
    Changed phenylalanine 74 into an alanine (F74A).
    Promoter
    ScADH1
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204602-plasmid-map-sequence-id-417406

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  20. pDEST14-DoubleCatcher-⍺-Lock

    Plasmid
    #216286
    Purpose
    Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Publication
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher ⍺-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    216286-plasmid-map-sequence-id-433335

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Showing: 11701 - 11720 of 33539 results