34,239 results
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Plasmid#232200PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
ExpressionBacterialAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
WNV isolate WN NY 2000-crow3356 NS5 RdRp full length
Plasmid#228662PurposeBacterial expression of the West Nile virus NS5 RdRp protein for biochemical assayDepositorInsertWest nile NS5 RdRp
TagsHis-SUMOExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus D68 (EV-D68) strain EV-D68 STL 2014 12 3C protease
Plasmid#228644PurposeBacterial expression of the D68 3C proteaseDepositorInsertEnterovirus D68 3C protease
TagsHis-SUMOExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease
Plasmid#228637PurposeBacterial expression of the Coxsackievirus 16 3C proteaseDepositorInsertCoxsackievirus 16 3C protease
TagsHis-SUMOExpressionBacterialAvailable sinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease
Plasmid#228636PurposeBacterial expression of the A71 3C protease (isoform B) used in crystallisationDepositorInsertEnterovirus A71 3C protease isoform B
TagsHis-SUMOExpressionBacterialAvailable sinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMVRha
Plasmid#229553PurposeBroad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-SisTnpB1–ωRNA
Plasmid#228365PurposeExpresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cellsDepositorInsertSisTnpB1
ExpressionBacterialPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-303_Cacnes_tetR
Plasmid#225618PurposeC. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherryDepositorInsertTetR
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-304_Cacnes_tetR
Plasmid#225619PurposeC. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherryDepositorInsertTetR
UseSynthetic BiologyTags6xHis and mCherry-V5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-224_Cacnes_sfGFP
Plasmid#225611PurposeReplicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.DepositorInsertsfGFP
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+) pCopA RBS30 mscarlet
Plasmid#226370PurposePlasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30DepositorInsertmScarlet
Tags6x HistagExpressionBacterialPromotercopA promoter (pCopA)Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry_YafQ/DinJ
Plasmid#229148PurposeToxin-Antitoxin (Dinj/YafQ)DepositorInsertmCherry, DinJ/YafQ
ExpressionBacterialPromoterPtlpA (mCherry), DinJ/YafQ (Native)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-MjCouRS
Plasmid#229066PurposetRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codonDepositorInsertMethanococcus jannaschii tRNA synthetase
ExpressionBacterialPromoterT7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSB19-PapRS#34-OmpA
Plasmid#231105PurposeVector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)DepositorTypeEmpty backboneExpressionBacterialAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28A-Rab8A
Plasmid#228880PurposeExpression of His-tagged Rab8A in E. coliDepositorInsertRAB8A (RAB8A Human)
TagsHis tagExpressionBacterialMutationresidues 1-6 and 177-207 missingAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSFDUET-1_VNp-anti-Lysozyme fAb
Plasmid#214951PurposeBacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusionsDepositorInsertsVNp-anti-Lysozyme Fab heavy chain fusion
VNp-anti-Lysozyme Fab light chain fusion
TagsVNpExpressionBacterialPromoterT7Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a_LYN
Plasmid#224238PurposeExpression of human LYN Kinase in E. coliDepositorAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA516
Plasmid#231118PurposeFragmid fragment: (2A Selection) CBE activity-based selection intronDepositorInsertPuroN'-intron-PuroC'
UseCRISPRExpressionBacterialAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA568
Plasmid#231117PurposeFragmid fragment: (guide cassette) CBE activity-based selection cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-14
Plasmid#228994PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-14
Tags6xHISExpressionBacterialPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only