32,457 results
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Plasmid#111253PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptideDepositorInsertTorA signal peptide
ExpressionBacterialPromoterPcAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pKS011
Plasmid#65464PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherryDepositorInsertMBP-Ntag-mCherry
TagsFLAG and MBPExpressionBacterialPromoterpl-TetOAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
cp196V1.2
Plasmid#52100PurposeA improved cpmCherry variantDepositorInsertcp196V1.2
Tags6 His tagExpressionBacterialPromoterpBAD promotorAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(400-772)
Plasmid#171087PurposeCo-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorTagsHisExpressionBacterialMutationDeleted N-term 26+399 amino acids for mutant expr…PromoterT5 promoter and T7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-STING(pLxIS)
Plasmid#221333PurposeOverexpression of STING CTT in bacteriaDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3-LYS-IV
Plasmid#218119PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKM2 WT
Plasmid#107158PurposeMBP-rPKM2 WT for production and purification of rat PKM2 WTDepositorInsertMBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
TagsC-term 6-his and N-term MBPExpressionBacterialPromoterPBADAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2-TP53
Plasmid#111089PurposepET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.DepositorInsertTP53 (TP53 Human)
ExpressionBacterialAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCoofy22
Plasmid#43992DepositorTypeEmpty backboneTagsHis10 and SExpressionBacterialPromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMM579
Plasmid#68882PurposeNanoLuc expressed constiutively from P1, pNBU2 backbone, AmpRDepositorInsertNanoLuc
ExpressionBacterialAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSMART-Mev1
Plasmid#65815PurposeNADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli ExpressionDepositorInsertsmvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
mvaS: hydroxymethylglutaryl-CoA synthase
ExpressionBacterialPromoterinsulated mipAp from E. coli and insulated waaHp…Available SinceSept. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SPASTIN , residues 112-196
Plasmid#180627PurposeBacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.DepositorInsertSPASTIN
TagsHIS-SUMOExpressionBacterialMutationResidues 112-196PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOSV808
Plasmid#126601Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
RGD-sfGFP-I27-CC
Plasmid#85392PurposeMTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labelingDepositorInsertRGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
Tags6x HisExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
5407 pClpB
Plasmid#1235DepositorInsertClpB
ExpressionBacterialMutationnoneAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-tLOV
Plasmid#100574PurposeExpression plasmid for the thermostable anaerobic fluorescent protein tLOVDepositorInserttLOV
Tags6xHisExpressionBacterialPromoterpTrcAvailable SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGMEH-T38M-750-luxAB-DAS+4
Plasmid#49520PurposeHygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomallyDepositorInsertT38M-P750-luxAB-DAS+4
TagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-HF1
Plasmid#218164PurposeThis plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG-HF1
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only