1,748 results

1. pJH2037

   Plasmid

   #179220

   Purpose

   Psra-11::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherry

   Depositor

   Mei Zhen

   Article

   Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

   Insert

   UNC-7 (unc-7 Nematode)
   

   Use

   Tags

   wCherry

   Expression

   Worm

   Mutation

   Promoter

   Psra-11

   Available since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK2067

   Plasmid

   #171845

   Purpose

   C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)

   Depositor

   Judith Kimble

   Article

   Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:

   Insert

   his-58, eGFP, mCherry
   

   Use

   Tags

   N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…

   Expression

   Worm

   Mutation

   boxB wild type and hairpin mutant

   Promoter

   mex-5 promoter

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDD398

   Plasmid

   #91832

   Purpose

   AID NT-tag linker donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Auxin-inducible degron (AID)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMNK55 - piRNAi 21ur-1224 - cku-80

   Plasmid

   #159820

   Purpose

   piRNA-induced RNA interference (piRNAi) to bias double-strand repair toward homologous recombination

   Depositor

   Christian Froekjaer-Jensen

   Article

   Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

   Insert

   cku-80 piRNAi 21ur-1224
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMNK54 - piRNAi 21ur-1224 - him-5

   Plasmid

   #159818

   Purpose

   piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

   Depositor

   Christian Froekjaer-Jensen

   Article

   Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

   Insert

   him-5 piRNAi 21ur-1224
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMNK56 - piRNAi 21ur-1224 - him-8

   Plasmid

   #159819

   Purpose

   piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

   Depositor

   Christian Froekjaer-Jensen

   Article

   Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

   Insert

   him-8 piRNAi 21ur-1224
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSR06

   Plasmid

   #69152

   Purpose

   read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr II

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Inserts

   mCherry
   eGFP
   

   Use

   Cre/Lox; Mossci

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0 and codon-optimzed index…

   Promoter

   rps-27

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCFJ150-cul4

   Plasmid

   #80353

   Purpose

   Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjection

   Depositor

   Sean Ryder

   Article

   Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

   Insert

   Pmex-5:GFP::H2B::PEST::cul-4 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCM5.52

   Plasmid

   #19381

   Depositor

   Geraldine Seydoux

   Article

   Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

   Insert

   him-3 3'utr in pDONRP2RP3 (him-3 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMLS254

    Plasmid

    #73721

    Purpose

    SapTrap Halotag + Cbr-unc-119 donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Halotag + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJW1927

    Plasmid

    #163093

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJW1886

    Plasmid

    #154333

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TC690

    Plasmid

    #122619

    Purpose

    Punc4::QF2::QS::mKate

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    QF2::QS::mKate
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

    Plasmid

    #182354

    Purpose

    Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

    Plasmid

    #182356

    Purpose

    Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPD93_97

    Plasmid

    #1476

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    myo3-2341, myo3Tsa, IVSA, SV40NLS, gfpN, IVSR, gf…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]

    Plasmid

    #159795

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ2214 - Intron GG1 - 900 bp

    Plasmid

    #159880

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG1 - 900 bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJH3558

    Plasmid

    #179611

    Purpose

    Punc-4::Chrimson::Cherry unc-54 3' UTR C.elegans A-MNs and others expression of Chrimson::Cherry

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    Chrimson
    

    Use

    Tags

    Cherry

    Expression

    Worm

    Mutation

    Promoter

    Punc-4

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCZGY3195

    Plasmid

    #172695

    Purpose

    Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    Punc-25
    

    Use

    Tags

    GFP 1-10

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge