8,058 results

1. pKT0090

   Plasmid

   #8714

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yEVenus
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   yEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS416-Aga1p-FAPB2.3.6L1TAG

   Plasmid

   #158148

   Purpose

   Yeast display reporter for non-display yeast strains (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p-FAPB2.3.6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first position of th…

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK59-ATG17-GFP

   Plasmid

   #44175

   Depositor

   James Hurley

   Article

   Ragusa et al Cell. 2012 Dec 21;151(7):1501-12. doi: 1

   Insert

   Atg17 (ATG17 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   none

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

4. PhyB-mCherry-CDC10

   Plasmid

   #51568

   Purpose

   Constitutively express bud neck localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   CDC10 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV0710

   Plasmid

   #133517

   Purpose

   Expression of mCherry tagged beta-tubulin to monitor microtubules

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(atb2)-mCherry-atb2-terminator(atb2)-hphMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDH22

   Plasmid

   #83798

   Purpose

   Useful for tagging yeast proteins with N-terminal YFP tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   YFP
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. plIVMD156

   Plasmid

   #160934

   Purpose

   In vivo mRNA display Gateway Destination construct

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

   Insert

   MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
   

   Use

   Tags

   HIS tag, 3'UTR MS2 Stem Loop

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG415GPD-ccdB-HA

   Plasmid

   #14242

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH728-CEN-RLuc/maxCFLuc

   Plasmid

   #38212

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD6-pGRR-r11-r10-yeGFP

    Plasmid

    #74368

    Purpose

    pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    yeGFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGRR-r11-r10

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA-MNase-CaARG4

    Plasmid

    #162463

    Purpose

    DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4

    Depositor

    Adnane Sellam

    Article

    Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

    Insert

    3FLAG-Mnase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMZ222

    Plasmid

    #52223

    Purpose

    Constitutive expression of humanized Cas9

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    silent mutation in the original CspCI site

    Promoter

    adh1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS403red-­luxTA2-­4F

    Plasmid

    #166030

    Purpose

    Expresses luxTA(2-­4F) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    luxR
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    S116Y, W201R, codon-optimized for expression in y…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS405red-­phlTA2-­1E

    Plasmid

    #166029

    Purpose

    Expresses phlTA(2-­1E) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    phlF
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG306-pTet07-synYFP[TTG/AGA]-nLuc

    Plasmid

    #199756

    Purpose

    Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-nLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMC-X4

    Plasmid

    #176154

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMC-XII1

    Plasmid

    #176159

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4046323

    Plasmid

    #149100

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMC-XI3

    Plasmid

    #176157

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUB23-M-beta-gal

    Plasmid

    #8816

    Depositor

    Alexander Varshavsky

    Article

    Bachmair et al Science 1986 Oct 10;234(4773):179-86.

    Insert

    beta-gal
    

    Use

    Tags

    Ubiquitin

    Expression

    Yeast

    Mutation

    M (methionine) at amino terminus of beta-gal

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only