8,418 results

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pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER

Plasmid

#52686

Purpose

Same as 41024, with additional linker

Depositor

Michael Nick Boddy

Insert

tetO7-Pcyc1-3xHA+LINKER

Use

Promoter replacement

Expression

Yeast

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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D588 lys2::HIS3 Disruptor Converter

Plasmid

#51675

Purpose

Yeast marker swap plasmid for converting LYS2 to HIS3

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

HIS3 (HIS3 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pDBD-M

Plasmid

#50572

Purpose

Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571

Depositor

Alexander Varshavsky

Publication

Turner et al Science. 2000 Sep 22;289(5487):2117-20.

Insert

DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin

Expression

Yeast

Promoter

Gal1

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only
pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Publication

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHH

Plasmid

#51057

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

Depositor

Yunde Zhao

Publication

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pYEXNHG3

Plasmid

#49025

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Publication

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMC6-TEFkFLP(LYS2)

Plasmid

#49458

Purpose

overespression of Flp step-arrest mutant in yeast, LYS2 selection

Depositor

Marc Gartenberg

Insert

Flp(H305L) (flp Budding Yeast)

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

TEF1

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pCH001

Plasmid

#47953

Purpose

tetO-GFP reporter casette

Depositor

Attila Becskei

Publication

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

GFP

Use

Tet inducible expression

Expression

Yeast

Promoter

T3

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pAAH2

Plasmid

#41826

Purpose

Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.

Depositor

Melissa Moore

Publication

Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Type

Empty backbone

Use

Pcr template

Expression

Bacterial and Yeast

Promoter

T7, TEF

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pJK59-ATG18-GFP

Plasmid

#42529

Depositor

James Hurley

Publication

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

Atg18 (ATG18 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

none

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pJK59-ATG17-GFP

Plasmid

#44175

Depositor

James Hurley

Publication

Ragusa et al Cell. 2012 Dec 21;151(7):1501-12. doi: 1

Insert

Atg17 (ATG17 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

none

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
YCplac111-yVps23-mCherry

Plasmid

#45925

Depositor

James Hurley

Publication

Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

Insert

yeast Vps23 (STP22 Budding Yeast)

Tags

mCherry

Expression

Yeast

Promoter

lac

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pETCON-DnvLB16

Plasmid

#45124

Depositor

David Baker

Publication

Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

Insert

DnvLB16

Tags

Aga2p for yeast surface display and c-myc epitope…

Expression

Yeast

Promoter

GAL

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomApple-CaURA3

Plasmid

#44879

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Expression

Yeast

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDN-G1GZmbh

Plasmid

#44516

Depositor

Gabor Balazsi

Publication

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
yEGFP::ZeoR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-NG1Tt

Plasmid

#44523

Depositor

Gabor Balazsi

Publication

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1 (wt) promoter (GAL1 Budding Yeast)
tetR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Promoter

PGal1 (wt)

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-T2dGZmxh

Plasmid

#44552

Depositor

Gabor Balazsi

Publication

Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

Inserts

PTETREG promoter
yEGFP::ZeoR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Promoter

PTETREG

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pETcon(I-LtrI)

Plasmid

#41523

Depositor

Andrew Scharenberg

Insert

I-LtrI

Tags

3x(G4S) Linker, G3S Linker, HA Tag, and Myc Tag

Expression

Yeast

Promoter

GAL1

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCP5b

Plasmid

#37185

Depositor

Adam Bogdanove

Publication

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Type

Empty backbone

Expression

Yeast

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pRS414-GPD-6xHis-Hsp82

Plasmid

#41188

Depositor

Dan Bolon

Publication

Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

Insert

S. cerevisiae Hsp82

Tags

6xHis

Expression

Yeast

Promoter

GPD

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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