8,086 results

1. YIp LYS2-HIS3

   Plasmid

   #122908

   Purpose

   LYS2-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYSD153

   Plasmid

   #212929

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYSD141

   Plasmid

   #212928

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS313_ATP3

   Plasmid

   #120253

   Purpose

   Expression of ATP3 in yeast

   Depositor

   Markus Ralser

   Article

   Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

   Insert

   ATP3 (ATP3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ATP3

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS313_ATP3T911A

   Plasmid

   #120254

   Purpose

   Expression of ATP3-6 in yeast

   Depositor

   Markus Ralser

   Article

   Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

   Insert

   ATP3 (ATP3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T911 changed to A; Isoleucine 304 changed to Aspa…

   Promoter

   ATP3

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS403red-­luxTA2-­4F

   Plasmid

   #166030

   Purpose

   Expresses luxTA(2-­4F) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   luxR
   

   Use

   Synthetic Biology

   Tags

   Nuclear localization signal and three copies of V…

   Expression

   Yeast

   Mutation

   S116Y, W201R, codon-optimized for expression in y…

   Promoter

   pPGK1 mutant

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS405red-­phlTA2-­1E

   Plasmid

   #166029

   Purpose

   Expresses phlTA(2-­1E) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   phlF
   

   Use

   Synthetic Biology

   Tags

   Nuclear localization signal and three copies of V…

   Expression

   Yeast

   Mutation

   P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

   Promoter

   pPGK1 mutant

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB3034(X-3 MarkerFree)

   Plasmid

   #73272

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMZ288

   Plasmid

   #59897

   Purpose

   Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   Available Since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMC-X4

    Plasmid

    #176154

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMC-XII1

    Plasmid

    #176159

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG416-GAL-TDP-43

    Plasmid

    #181709

    Purpose

    Galactose inducible expression of TDP-43

    Depositor

    Alejandro Chavez

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pILAC3

    Plasmid

    #185890

    Purpose

    In vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    see comments
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 F96C; Xd.CRtYB E83K

    Promoter

    Available Since

    Sept. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pIT6EG7ml

    Plasmid

    #185886

    Purpose

    In vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (14 copy).

    Depositor

    Claudia Vickers

    Insert

    see comments
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 F96W N127W

    Promoter

    Available Since

    Sept. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pMC-XI3

    Plasmid

    #176157

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPICZ-mouseGIRK2-eGFP-StII-6xHis

    Plasmid

    #124277

    Purpose

    Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

    Depositor

    Arthur Laganowsky

    Article

    Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

    Insert

    GIRK2 (Kcnj6 Mouse)
    

    Use

    Tags

    eGFP-StreptagII-6xHis

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. G31, Cdc20-AID tagging

    Plasmid

    #130269

    Purpose

    Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

    Depositor

    Matthias Heinemann

    Article

    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

    Insert

    Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
    

    Use

    Tags

    AID(71-114)

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. HIP2

    Plasmid

    #31436

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Huntingtin Interacting protein 2 (UBE2K Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pPS811

    Plasmid

    #8904

    Depositor

    Pamela Silver

    Article

    Lee et al Genes Dev 1996 May 15;10(10):1233-46.

    Insert

    NPL3 (NPL3 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pICE HIS3

    Plasmid

    #122877

    Purpose

    HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge