8,058 results
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YIplac211-GGA2-msGFPx3
Plasmid#105258PurposePlasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.DepositorInsertGga2 portion (GGA2 Budding Yeast)
UseTagsmsGFPx3ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFP97
Plasmid#83551PurposeUsed to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.DepositorInsertCYC1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCYC1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET16
Plasmid#107442PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET16 (MET16 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-F1-empty vector
Plasmid#125551PurposeN2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 alone without any attached prot…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
YL-CashGem-BAT1-URA3-LINEAR
Plasmid#174837PurposepRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolyticaDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterGPDpAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
p415PGK1-AtMAX1(pYL759)
Plasmid#178289PurposeExpresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5DepositorInsertAtMAX1
UseTagsExpressionYeastMutationPromoterPGK1Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPMVAugw
Plasmid#98300PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.DepositorInsertTEFM1EfmvaE>TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVG49
Plasmid#164707PurposeyEVenus under the control of GAL1 promoterDepositorInsertGAL1pr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP-TAG
Plasmid#158131PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)DepositorInsertsfGFP
UseTagsExpressionYeastMutationIntroduced a stop codon in place of the tyrosine …PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pRS426-TUB1
Plasmid#60385PurposeExpression of yeast alpha tubulin (TUB1) using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#64177Purposeharbours marker gene LEU2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS423-FjTAL(JBEI-15466)
Plasmid#87936PurposeExpression in S.cerevisiae of FjTALDepositorInsertFjTAL
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-G11-5FLAG-kanMX6
Plasmid#49182Purposegenomic G11-5FLAG epitope tagging in S. pombeDepositorTypeEmpty backboneUseTagsG11-5FLAGExpressionYeastMutationPromoterAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-Purg1-3FLAG
Plasmid#19355DepositorInserturg1 promoter
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
416GPD-FUS-YFP
Plasmid#29594DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceJuly 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ENT5-HaloTag
Plasmid#177102PurposeExpresses Ent5-HaloTag in yeast cellsDepositorInsertENT5 (ENT5 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Htb2
Plasmid#51569PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsHTB2 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymNeongreen-T2A-mTurquoise2
Plasmid#125701PurposeProduces equimolar expression of yeast codon optimized mNeongreen and mTurquoise2DepositorInsertymNeongreen-T2A-mTurquoise
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only