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YIp128-P30-His-pol30(K164R)

Plasmid

#99544

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K164R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAW

Plasmid

#64745

Purpose

Yeast expression plasmid: ACC1

Depositor

Jens Nielsen

Article

Shi et al MBio. 2014 May 6;5(3):e01130-14. doi: 10

Insert

ACC1

Expression

Yeast

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1

Plasmid

#60385

Purpose

Expression of yeast alpha tubulin (TUB1) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pAG425GPD-Cerulean-ccdB

Plasmid

#14418

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Cerulean

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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FC858

Plasmid

#27328

Depositor

Fred Cross

Article

Cross et al Yeast. 2011 Feb . 28(2):167-79.

Insert

CDH1 (CDH1 Budding Yeast)

Expression

Yeast

Mutation

11 Phosphorylation site point mutations: T12A S16…

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pAG305GAL-ccdB-EGFP

Plasmid

#14185

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pAV0758

Plasmid

#133511

Purpose

Expression of 3GFP tagged RasAct probe to monitor active Ras1

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)

Expression

Yeast

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0783

Plasmid

#133515

Purpose

Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pRS415-KEX2

Plasmid

#10996

Depositor

Francisco Malagon

Insert

KEX2 (KEX2 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Dec. 29, 2005

Availability

Academic Institutions and Nonprofits only

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APEX2 VLP

Plasmid

#182211

Purpose

Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).

Depositor

Claudia Vickers

Inserts

VP2C-APEX2
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology; Tem imaging

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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303Gal-FUS

Plasmid

#29614

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Expression

Yeast

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
pDR FLIP43 GPS1-NR

Plasmid

#101085

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1-NR

Tags

6HIS and cMyc

Expression

Yeast

Mutation

AtGAI E51K E54R, AtGID1C S114A F115A

Promoter

pPMA1

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDS1209

Plasmid

#177340

Purpose

Expresses Aequorin in Candida albicans using the URA3 auxotrophic marker URA3

Depositor

Dominique Sanglard

Article

Sanglard et al J Fungi (Basel). 2021 Apr 20;7(4). pii:

Insert

Aequorin

Expression

Yeast

Promoter

ACT1

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMEL15

Plasmid

#107921

Purpose

nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046494

Plasmid

#149137

Purpose

Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 surface glycoprotein (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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cyterm-ymtq2 pDRF1-GW

Plasmid

#168083

Purpose

Expresses Cyterm-ymTq2 to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-ymTq2

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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BS-Leu2

Plasmid

#69200

Purpose

complementation of yeast auxotrophic marker LEU2

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

LEU2

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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YIPlac204-TC-Sec7-6xDsRed-M1

Plasmid

#25448

Depositor

Benjamin Glick

Article

Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

Insert

Sec7 (SEC7 Budding Yeast)

Tags

6xDsRed.M1

Expression

Yeast

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pRG213MX

Plasmid

#64522

Purpose

Centromeric E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pGAL-SKI1-NAT

Plasmid

#160382

Purpose

Overxpression of SKI1/XRN1 in yeast

Depositor

John McCusker

Insert

SKI1

Expression

Yeast

Promoter

GAL

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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