8,086 results

1. pFA6a-6xGLY-T7-HIS3MX6

   Plasmid

   #20759

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS-Dhh1-PA (405)

   Plasmid

   #121417

   Purpose

   Expressing Dhh1-PA

   Depositor

   Daniel Klionsky

   Article

   Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

   Insert

   Dhh1promoter-Dhh1 ORF-PA-ADH1 terminator (DHH1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG306GAL-ccdB-HA

   Plasmid

   #14235

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. YIplac211-ARF1-msGFP

   Plasmid

   #105255

   Purpose

   Plasmid for pop-in/pop-out replacement of Arf1 with Arf1-msGFP.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Arf1 (ARF1 Budding Yeast)
   

   Use

   Tags

   msGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ cMyc Dam_f_GFPoNLS

   Plasmid

   #85820

   Purpose

   iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   cMyc Dam_f_GFPoNLS
   

   Use

   Tags

   cMyc and oNLS (optimized nuclear localization sig…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A.

   Promoter

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS416 Gal TDP43 Q331K

   Plasmid

   #27461

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Glutamine 331 mutated to Lysine (Q331K)

   Promoter

   Available Since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pNS1

   Plasmid

   #131769

   Purpose

   PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   FUSN_Fusion Red_Cry2WT
   

   Use

   Integration into δ-sites

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PADH1

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HCP5

   Plasmid

   #166107

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.

   Depositor

   Matthias Heinemann

   Insert

   Cyr1CT-sgRNA (CYR1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIplac211-iGFP-TLG2

   Plasmid

   #105262

   Purpose

   Plasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Tlg2 (TLG2 Budding Yeast)
   

   Use

   Tags

   iGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTH734-2µ-RLuc/stopCFLuc

    Plasmid

    #40609

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase (nonsense mutant)
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDH18

    Plasmid

    #83803

    Purpose

    YFP-CFP positive FRET control; his selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    YFP-CFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKT0103

    Plasmid

    #8718

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yEVenus
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …

    Promoter

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTY24

    Plasmid

    #83794

    Purpose

    Useful for tagging yeast proteins with C-terminal DsRed tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    DsRed
    

    Use

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYC52

    Plasmid

    #63908

    Purpose

    Recipient integrative vector to construct CFP tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG306GPD-ccdB-HA

    Plasmid

    #14236

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIYC05

    Plasmid

    #64742

    Purpose

    Yeast expression plasmid: acsL641P, ALD6

    Depositor

    Jens Nielsen

    Article

    Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

    Insert

    acsL641P, ALD6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

17. GTH-r

    Plasmid

    #81106

    Purpose

    Gene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    mCherry
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBT metaxin 2

    Plasmid

    #12419

    Depositor

    Paul Bornstein

    Insert

    metaxin 2 (Mtx2 Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 23, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pFS119

    Plasmid

    #12379

    Depositor

    Nick Rhind

    Article

    Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

    Insert

    ade6, thymidine kinase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. LD211

    Plasmid

    #69183

    Purpose

    Integration of 2GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg11-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge