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pSC6

Plasmid

#233020

Purpose

Inducible Conjugative Plasmid

Depositor

Bogumil Karas

Publication

Jaafar et al Can J Microbiol. 2025 May 6;71:1-9. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pFA6a-mIAA7-ALFA-HIS3MX6

Plasmid

#247627

Purpose

Yeast gene tagging with mIAA7

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

mIAA7

Tags

ALFA

Expression

Yeast

Available since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-mIAA7-3xFLAG-kanNP1

Plasmid

#247620

Purpose

Yeast gene tagging with mIAA7

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

mIAA7

Tags

3xFLAG

Expression

Yeast

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pNH605-Padh-OsTIR1(F74A)

Plasmid

#247610

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74A

Promoter

ADH1

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pNH605-Padh-OsTIR1(F74G)

Plasmid

#247605

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pADH100Cau

Plasmid

#244817

Purpose

Modified plasmid from pADH100 to perform HIS-FLP CRISPR in Candidozyma auris

Depositor

Patrick Van Dijck

Publication

Sofras et al bioRxiv [Preprint]. 2025 Jan 11:2025.01.

Insert

SNR52p from C. auris

Use

CRISPR

Tags

HIS1 terminator from C. auris and NAT resistance …

Expression

Yeast

Promoter

SNR52p

Available since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pADH99Cau

Plasmid

#244807

Purpose

Modified plasmid from pADH99 to perform HIS-FLP CRISPR in Candidozyma auris

Depositor

Patrick Van Dijck

Publication

Sofras et al bioRxiv [Preprint]. 2025 Jan 11:2025.01.

Insert

CauHIS1_US

Use

CRISPR

Tags

C. albicans MAL2 promoter, C. albicans codon opti…

Expression

Yeast

Available since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2678

Plasmid

#241723

Purpose

Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BLE marker for selection on phleomycin

Depositor

Hiten Madhani

Publication

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pFS554

Plasmid

#242854

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW3 promoter at its endogenous locus

Depositor

Nick Rhind

Publication

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW3 promoter

Available since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pACT-HAB1

Plasmid

#241276

Purpose

Yeast two hybrid vector expressing AD-HAB1

Depositor

Sean Cutler

Publication

Park et al Science. 2009 May 22;324(5930):1068-71.

Insert

HAB1 (HAB1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFS553

Plasmid

#242853

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW2 promoter at its endogenous locus

Depositor

Nick Rhind

Publication

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW2 promoter

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDRF1 ymBaojin-T2A-mCherry

Plasmid

#219857

Purpose

equimolar expression of ymBaojin and mCherry in budding yeast

Depositor

Bas Teusink

Publication

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

ymBaojin-T2A-mCherry

Expression

Yeast

Available since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLS416

Plasmid

#239127

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3

Depositor

Sahand Jamal Rahi

Publication

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pJRoC30-LaccID

Plasmid

#234652

Purpose

Expresses LaccID evolved variant in yeast

Depositor

Miguel Alcalde Galeote, Alice Ting

Publication

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LaccID

Tags

HRV 3C protease site-GSG linker-8xHis tag

Expression

Yeast

Mutation

V11A, Q45R, N74S, R121S, I126V, D255G, L300V

Promoter

Gal1p

Available since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPQ13BE5

Plasmid

#223836

Purpose

Expressing a cytosolic cAMP sensor (mScarletI3-ApacH-StayGold2) in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Publication

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mScarletI3>ApacH>StayGold2

Expression

Yeast

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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Sc [Pol31 + Pol32]-pRS403/GAL

Plasmid

#239199

Purpose

Overerxpress Sc Pol31 & Pol32 when integrated into yeast (S. cer)

Depositor

Michael O'Donnell

Inserts

Pol31 (POL31 Budding Yeast)
Pol32 (POL32 Budding Yeast)

Expression

Yeast

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Sc Pol3-FLAG(Ec)-pRSF405/GAL

Plasmid

#239198

Purpose

Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)

Depositor

Michael O'Donnell

Insert

Pol3 (POL3 Budding Yeast)

Tags

3X FLAG

Expression

Yeast

Mutation

E. coli codon bias (synthetic gene), but same ami…

Available since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLS410

Plasmid

#239123

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX

Depositor

Sahand Jamal Rahi

Publication

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAL003

Plasmid

#233864

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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P415 ymBaojin

Plasmid

#224086

Purpose

Expressing ymBaojin in yeast

Depositor

Bas Teusink

Insert

ymBaoJin

Expression

Yeast

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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