8,265 results
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Plasmid#133903PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only
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pDH18
Plasmid#83803PurposeYFP-CFP positive FRET control; his selectionDepositorInsertYFP-CFP
ExpressionYeastAvailable SinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-P81nmt1-3FLAG
Plasmid#19350DepositorInserthphMX6
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
M3499 ura3::ADE2 Disruptor Converter
Plasmid#51674PurposeYeast marker swap plasmid for converting URA3 to ADE2DepositorAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXP414
Plasmid#26841DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAG413GAL-ccdB-TAP
Plasmid#14261DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTY24
Plasmid#83794PurposeUseful for tagging yeast proteins with C-terminal DsRed tag; G418 selectionDepositorInsertDsRed
TagsDsRedExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSCupGFP
Plasmid#1086DepositorInsertGFP
ExpressionYeastAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pICXC3H
Plasmid#49022PurposeFX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tagDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionYeastPromoterAOX1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-TLG2
Plasmid#105262PurposePlasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.DepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH3FS-PYK1
Plasmid#85781PurposeN-ICE plasmid pH3FS-PYK1DepositorInsertloxP-HphMX-loxP-PYK1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCHAP4
Plasmid#206850PurposeA plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pV1464
Plasmid#111440PurposeN. castellii Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDA200
Plasmid#74248PurposepGPD1-dPSTRy(4,3)DepositorInsertpGPD1-dPSTRy(4,3)
ExpressionBacterial and YeastAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDBD-R
Plasmid#50571PurposeDouble Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572DepositorInsertDHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
ExpressionYeastMutationM1R (B-gal)PromoterGal1Available SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ARF1-msGFP
Plasmid#105255PurposePlasmid for pop-in/pop-out replacement of Arf1 with Arf1-msGFP.DepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK59-ATG18-GFP
Plasmid#42529DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES
Plasmid#182485PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free PcPTS
Plasmid#182486PurposeYeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS-PTS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB2226
Plasmid#67533PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only