8,086 results

1. pXRL2

   Plasmid

   #63148

   Purpose

   Leu2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

   Depositor

   Marc Gartenberg

   Article

   Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS314-prp16-302

   Plasmid

   #89974

   Purpose

   prp16-302 allele in TRP plasmid

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP16 (PRP16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   prp16-302 allele (R456K and G691R)

   Promoter

   Available Since

   Aug. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RB793

   Plasmid

   #199121

   Purpose

   This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome

   Depositor

   Richard Bennett

   Insert

   100 bp barcoding cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RB794

   Plasmid

   #199122

   Purpose

   This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome

   Depositor

   Richard Bennett

   Insert

   100 bp barcoding cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS303 prp8-101

   Plasmid

   #111307

   Purpose

   prp8-101

   Depositor

   Christine Guthrie

   Article

   Umen et al Genetics. 1996 Jun;143(2):723-39.

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   prp8-101 (E1960K)

   Promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSK260

   Plasmid

   #129252

   Purpose

   dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   Sup35 NM yTRAP sensor reporting on mKate2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHM4 (U6 in pSE360)

   Plasmid

   #111281

   Purpose

   U6 snRNA coding sequence in pSE360(Ura) plasmid

   Depositor

   Christine Guthrie

   Article

   Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

   Insert

   SNR6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pICE ADE2-HphMx

   Plasmid

   #122895

   Purpose

   ADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PhyB-mCherry-SNF7

   Plasmid

   #51574

   Purpose

   Constitutively express endosome localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   SNF7 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LHP416-PAN1-HA

    Plasmid

    #32159

    Depositor

    Linda Hicke

    Insert

    PAN1 (PAN1 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. YIplac211-msGFP2-STE13

    Plasmid

    #177106

    Purpose

    Expresses msGFP2-Ste13 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    STE13 (STE13 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. YIplac204-pOst1-ESCargo*

    Plasmid

    #115427

    Purpose

    Expresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    pOst1-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSD02

    Plasmid

    #16009

    Depositor

    Fred Cross

    Article

    Di Talia et al Nature. 2007 Aug 23. 448(7156):947-51.

    Insert

    ACT1pr-DsRed.T1.N1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYES2.1-cerMET2

    Plasmid

    #107435

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET2 (MET2 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pITGFP89

    Plasmid

    #83560

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter -yEGFP-CLN2PEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMZ289

    Plasmid

    #59898

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPS808

    Plasmid

    #8856

    Depositor

    Pamela Silver

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pAS2

    Plasmid

    #12389

    Depositor

    Nick Rhind

    Article

    Sigova et al Genes Dev. 2004 Oct 1. 18(19):2359-67.

    Insert

    green fluorescent protein
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GAL 103Q+ProCFPp303

    Plasmid

    #15575

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt103Q + Pro (HTT Human)
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pJZC593

    Plasmid

    #62319

    Purpose

    sgRNA with MS2-PP7 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + MS2-PP7 RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge