8,086 results

1. pKT0174

   Plasmid

   #8720

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yECFP
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   yEGFP with F64L, S65T, Y66W, N144I, M153T, V163A

   Promoter

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4046437

   Plasmid

   #145415

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pZ_P-GAP-ACS1

   Plasmid

   #126712

   Purpose

   yeast plasmid complementing ACS1 from Komagataella phaffii

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   acs1
   

   Use

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAP

   Available Since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGWKS4

   Plasmid

   #139411

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mCherry marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB5935

   Plasmid

   #106128

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntA_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGBW-m4046431

   Plasmid

   #145526

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pAG305GAL-ccdB-HA

   Plasmid

   #14233

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNC820

   Plasmid

   #20383

   Depositor

   Beverly Errede

   Article

   Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

   Insert

   UAS cassette (FUS1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   X=UbiM (see schematic)

   Promoter

   Available Since

   March 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBW-m4046476

   Plasmid

   #145512

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGP8GF-ccdB

    Plasmid

    #188445

    Purpose

    Cloning Vector and pDEST

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    ccdB
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. HCP8

    Plasmid

    #166110

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5

    Depositor

    Matthias Heinemann

    Insert

    Whi5-sg106 (WHI5 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDN-T1GZmbh

    Plasmid

    #44522

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
    yEGFP::ZeoR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Use

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXP831

    Plasmid

    #46065

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046672

    Plasmid

    #153918

    Purpose

    Yeast expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pRG225

    Plasmid

    #64526

    Purpose

    Episomal E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB2192

    Plasmid

    #67539

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYES2.1-cerMET5

    Plasmid

    #107437

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET5 (MET5 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

    Promoter

    GAL1

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYES2.1-pomMet17

    Plasmid

    #107453

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met17 (met17 Fission Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS316-GFP-COF1

    Plasmid

    #31843

    Depositor

    David Drubin

    Article

    Okreglak et al J Cell Biol. 2007 Sep 24;178(7):1251-64.

    Insert

    Cofilin (COF1 Budding Yeast)
    

    Use

    Tags

    GFP (S65T)

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available Since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only