8,388 results

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NAT-TET-OFF -LEU- 4xFLAG

Plasmid

#182527

Purpose

Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

7 Copies of TetO Box

Tags

4XFLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT GFP CL1

Plasmid

#182512

Purpose

C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CL1 Degron

Tags

GFP

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTPI1-EMC1-GFP-APEX2-URA3

Plasmid

#184776

Purpose

Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

APEX2

Expression

Yeast

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Kanamycin

Plasmid

#188933

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Kanamycin

Expression

Yeast

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pILGFP4I

Plasmid

#104504

Purpose

Used to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SkGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4Q

Plasmid

#104512

Purpose

Used to evaluate the expression output of Saccharomyces kudriavzevii GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SkGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGAL-PDE1

Plasmid

#160388

Purpose

Overxpression of PDE1 in yeast

Depositor

John McCusker

Insert

PDE1

Expression

Yeast

Promoter

GAL

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-NAT-Ago-Dcr

Plasmid

#160381

Purpose

Overexpression of Ago-Dcr fusion protein in yeast

Depositor

John McCusker

Insert

Ago- Dcr

Expression

Yeast

Promoter

GAL

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Linked Free NES (M)

Plasmid

#182436

Purpose

Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Insert

FPPS(M)-NES

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL10

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 WT

Plasmid

#169261

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 WT (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Promoter

GPD

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Yeast_Target-AID3S

Plasmid

#188646

Purpose

Expresses Target-AID3S in yeasts

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Tags

UGI

Expression

Yeast

Mutation

D10A for SpCas9

Promoter

pGal1

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4L

Plasmid

#104507

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Nov. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pIAeBlueHPV16LR

Plasmid

#185894

Purpose

In vivo gene amplification (HapAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)

Expression

Yeast

Mutation

None

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Linked Free NES

Plasmid

#182485

Purpose

Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Insert

FPPS-NES

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL10

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Linked Free PcPTS

Plasmid

#182486

Purpose

Yeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Insert

FPPS-PTS

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL10

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Coexpressed Free GFP-NES

Plasmid

#182491

Purpose

Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Inserts

FPPS
GFP-NES

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL10 and GAL7

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.028

Plasmid

#184965

Purpose

Test effect of extending -Eco1 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(extended), a1/a2 length: 27

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3868

Plasmid

#185745

Purpose

Sub-array backbone 4/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW AKAR3-EV-NR

Plasmid

#182534

Purpose

Expresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeast

Depositor

Bas Teusink

Article

Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

Insert

AKAR3-EV-NR

Expression

Yeast

Mutation

T506A mutation

Promoter

PMA1

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGBKT7-RmMYB108

Plasmid

#173183

Purpose

Expresses Rosa multiflora MYB108 for transcriptional activation studies in yeast.

Depositor

Daidi Che

Article

Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

Insert

RmMYB108

Expression

Yeast

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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