8,430 results
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Plasmid#185894PurposeIn vivo gene amplification (HapAmp) of AeBlue + HPV16 L1 expression module at RPL25 locusDepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
ExpressionYeastMutationNoneAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Linked Free NES
Plasmid#182485PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free PcPTS
Plasmid#182486PurposeYeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS-PTS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL7 and GAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.028
Plasmid#184965PurposeTest effect of extending -Eco1 a1/a2 in yeastDepositorInsertEco1: RT and ncRNA(extended), a1/a2 length: 27
ExpressionYeastMutationhuman codon optimized RT, a1/a2 length extended t…PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS3868
Plasmid#185745PurposeSub-array backbone 4/4DepositorTypeEmpty backboneExpressionYeastAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW AKAR3-EV-NR
Plasmid#182534PurposeExpresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeastDepositorInsertAKAR3-EV-NR
ExpressionYeastMutationT506A mutationPromoterPMA1Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-RmMYB108
Plasmid#173183PurposeExpresses Rosa multiflora MYB108 for transcriptional activation studies in yeast.DepositorInsertRmMYB108
ExpressionYeastAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-mRuby2
Plasmid#176549PurposeExpression of mRuby2 for auxotrophic selection in the absence of uracilDepositorInsertyomRuby2
ExpressionYeastPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-Sapphire
Plasmid#176568PurposeExpression of Sapphire for auxotrophic selection in the absence of leucineDepositorInsertyETSapphire
ExpressionYeastPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHpgRNA50
Plasmid#160128PurposeTo express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1DepositorInsertPTEF1-HH-gRNA-LSC2- HDV-TAMO
ExpressionYeastAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- PGK1prom-TIR1-FLAG
Plasmid#189724PurposeEnables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)DepositorInsertOsTIR1
ExpressionYeastPromoterPGK1 promoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a mScarlet-FKBP::KANMX
Plasmid#185905PurposePlasmid for cloning mScarlet fused to FKBP into yeast.DepositorInsertmScarlet-FKBP
TagsmScarlet fused to FKBPExpressionYeastPromoterSP6Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- TEF2prom-TIR1F74G-FLAG
Plasmid#189727PurposeEnables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)DepositorInsertOsTIR1
TagsFLAGExpressionYeastPromoterTEF2Available sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- HTA1prom-TIR1 F74G -FLAG
Plasmid#189728PurposeEnables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)DepositorInsertOsTIR1
TagsFLAGExpressionYeastPromoterHTA1Available sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisSumoOphP
Plasmid#189672PurposeHisSUMO tagged OphP to express OphP in pichia pastorias.DepositorPublicationInsertHisSumoOphP
TagsHisSumo taggedExpressionYeastAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-HALO-GS
Plasmid#188940PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertpBS-SKII-URA-GAL1-HALO-GS
ExpressionYeastAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fCLIP-3xHA
Plasmid#188936PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fCLIP-3xHA
ExpressionYeastAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-NAT
Plasmid#188926PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-NAT
ExpressionYeastAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fCLIP-Kan
Plasmid#188923PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-Kan
ExpressionYeastAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only