8,060 results

1. pKT0212

   Plasmid

   #8725

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yECFP A206R
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…

   Promoter

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPTK011-3a-SA-αMFΔ

   Plasmid

   #84970

   Purpose

   Type 3a, Serum albumin followed by αMFΔ - secretion signal

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   Serum albumin followed by αMFΔ secretion tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Non

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046300

   Plasmid

   #149043

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGBW-m4046182

   Plasmid

   #149019

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCHFLAGGR

   Plasmid

   #1279

   Depositor

   Susan Lindquist

   Article

   Kimura et al Science 1995 Jun 2;268(5215):1362-5.

   Insert

   GR (Nr3c1 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pBW105

   Plasmid

   #111137

   Purpose

   For the expression of rat AANAT with Met-Leu-Ser N-terminus in S. cerevisiae

   Depositor

   Alexander Varshavsky

   Article

   Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

   Insert

   MLS-ratAANAT-3FLAG (Aanat Rat)
   

   Use

   Tags

   3x Flag

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG306GAL-ccdB-TAP

   Plasmid

   #14259

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   TAP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG413GAL-ccdB-ECFP

   Plasmid

   #14165

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   ECFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPTK019-4-tAOX1

   Plasmid

   #84988

   Purpose

   Type 4, tAOX, Alcohol oxidase 1 terminator

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   Alcohol oxidase 1 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Non

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAS2-1-Esf1

    Plasmid

    #99987

    Purpose

    Express yeast Esf1 with Y2H binding domain fusion

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Esf1 (ESF1 Budding Yeast)
    

    Use

    Tags

    Y2H binding domain

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL6 - Brr2 del247 WT

    Plasmid

    #90256

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

    Insert

    BRR2
    

    Use

    Tags

    TAP

    Expression

    Yeast

    Mutation

    deleted first 247 amino acids

    Promoter

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 416-Met25 Kar2 printor HDEL

    Plasmid

    #32129

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    Printor (KLHL14 Human)
    

    Use

    Tags

    HDEL and Kar2

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pRPB1-CTD14

    Plasmid

    #112822

    Purpose

    pSMF2 with 14 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDRF1-GW ymTurquoise2-T2A-mCherry

    Plasmid

    #118442

    Purpose

    Produces equimolar expression of yeast codon-optimized mTq2 and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. YIp ADE2-KanMx

    Plasmid

    #122917

    Purpose

    ADE2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCTD26-S>A10-17

    Plasmid

    #112826

    Purpose

    pSMF2 with all S to A mutations in only repeats 10-17.

    Depositor

    Stephen Fuchs

    Article

    Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    pSMF2 with all S to A mutations in only repeats 1…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCTD26-S>A2-9

    Plasmid

    #112825

    Purpose

    pSMF2 with all S to A mutations in only repeats 2-9.

    Depositor

    Stephen Fuchs

    Article

    Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    pSMF2 with all S to A mutations in only repeats 2…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCTD26-S>A18-25

    Plasmid

    #112827

    Purpose

    pSMF2 with all S to A mutations in only repeats 18-25.

    Depositor

    Stephen Fuchs

    Article

    Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    pSMF2 with all S to A mutations in only repeats 1…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LD224

    Plasmid

    #69191

    Purpose

    Integration of 2GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg24-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LD218

    Plasmid

    #69187

    Purpose

    Integration of 2GFP tag at Atg18 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg18-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge