8,060 results

1. pJZC548

   Plasmid

   #62316

   Purpose

   sgRNA with 1x PP7 for yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + 1x PP7
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPS2700

   Plasmid

   #8924

   Depositor

   Pamela Silver

   Article

   Lei et al Genes Dev. 2001 Jul 15. 15(14):1771-82.

   Insert

   YRA1 (YRA1 Budding Yeast)
   

   Use

   Tags

   3x Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pLexA::nud-1

   Plasmid

   #11339

   Depositor

   Guy Caldwell

   Insert

   nud-1 (nud-1 Nematode)
   

   Use

   Tags

   LexA DNA Binding Domain

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pLexA::lis-1

   Plasmid

   #11338

   Depositor

   Guy Caldwell

   Insert

   lis-1 (lis-1 Nematode)
   

   Use

   Tags

   LexA DNA Binding Domain

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pAG306GAL-EYFP-ccdB

   Plasmid

   #14331

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBW-m4046374

   Plasmid

   #149120

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGBW-m4046372

   Plasmid

   #149048

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 helicase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 helicase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4046194

   Plasmid

   #148979

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046521

   Plasmid

   #145473

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCfB2992

    Plasmid

    #63642

    Purpose

    pCfB2992 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG426GAL-ccdB-ECFP

    Plasmid

    #14179

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pILGFP84

    Plasmid

    #83542

    Purpose

    Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    SSA1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SSA1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCfB2228

    Plasmid

    #67540

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBW-m4046696

    Plasmid

    #153882

    Purpose

    Yeast expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pSP571

    Plasmid

    #139498

    Purpose

    CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pGPD Cas9 / sgRNA (STL162)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pGPD

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTH399-SUP45-S30P

    Plasmid

    #29372

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S30P mutant gene (corresponding to the sl23ts all…

    Promoter

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pTH375-SUP45-P174Q

    Plasmid

    #29383

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    P174Q mutant gene including genomic sequence 1432…

    Promoter

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

18. p2HGAL/GR/CYC

    Plasmid

    #1220

    Depositor

    Susan Lindquist

    Article

    Nathan et al Mol Cell Biol 1995 Jul;15(7):3917-25.

    Insert

    GR (Nr3c1 Rat)
    

    Use

    2micron

    Tags

    Expression

    Yeast

    Mutation

    pRS423 with GAL promoter and CYC terminator, 2mic…

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pFA6a-6xGLY-HSV-kanMX6

    Plasmid

    #20775

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNX3a-PK6

    Plasmid

    #78355

    Purpose

    fission yeast epitope tagging

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    6xPK
    kanMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge