8,133 results

1. 413-Met25 Kar2 TorsinA K108A GFP

   Plasmid

   #32125

   Depositor

   Susan Lindquist

   Article

   Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

   Insert

   TorsinA (TOR1A Human)
   

   Tags

   GFP, HDEL, and Kar2

   Expression

   Yeast

   Mutation

   K108A

   Available Since

   Nov. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

2. LD224

   Plasmid

   #69191

   Purpose

   Integration of 2GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg24-2EGFP
   

   Tags

   2xEGFP

   Expression

   Yeast

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LD218

   Plasmid

   #69187

   Purpose

   Integration of 2GFP tag at Atg18 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg18-2EGFP
   

   Tags

   2xEGFP

   Expression

   Yeast

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PSUC2-GFPantisense-CYC1t-pRS413

   Plasmid

   #64404

   Purpose

   SUC2 regulated expression of an antisense RNAi construct for GFP

   Depositor

   Claudia Vickers

   Article

   Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

   Insert

   GFPantisense
   

   Expression

   Yeast

   Promoter

   SUC2

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL6 - Brr2 del247 WT

   Plasmid

   #90256

   Purpose

   protein expression

   Depositor

   Christine Guthrie

   Article

   Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

   Insert

   BRR2
   

   Tags

   TAP

   Expression

   Yeast

   Mutation

   deleted first 247 amino acids

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRPB1-CTD14

   Plasmid

   #112822

   Purpose

   pSMF2 with 14 CTD repeats

   Depositor

   Stephen Fuchs

   Article

   Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

   Insert

   RPB1 (RPO21 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   CTD repeats have identical sequence, deleted repe…

   Promoter

   Native promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046300

   Plasmid

   #149043

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pGBW-m4046182

   Plasmid

   #149019

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBW105

   Plasmid

   #111137

   Purpose

   For the expression of rat AANAT with Met-Leu-Ser N-terminus in S. cerevisiae

   Depositor

   Alexander Varshavsky

   Article

   Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

   Insert

   MLS-ratAANAT-3FLAG (Aanat Rat)
   

   Tags

   3x Flag

   Expression

   Yeast

   Promoter

   Gal1

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG303GPD-ccdB-TAP

    Plasmid

    #14254

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Available Since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS2-1-Esf1

    Plasmid

    #99987

    Purpose

    Express yeast Esf1 with Y2H binding domain fusion

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Esf1 (ESF1 Budding Yeast)
    

    Tags

    Y2H binding domain

    Expression

    Yeast

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDRF1-GW ymTurquoise2-T2A-mCherry

    Plasmid

    #118442

    Purpose

    Produces equimolar expression of yeast codon-optimized mTq2 and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2-T2A-mCherry
    

    Expression

    Yeast

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2HGPD/ER/cyc

    Plasmid

    #1283

    Depositor

    Susan Lindquist

    Article

    Kimura et al Science 1995 Jun 2;268(5215):1362-5.

    Insert

    ER
    

    Tags

    Gpd promoter

    Expression

    Yeast

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

14. YIp ADE2-KanMx

    Plasmid

    #122917

    Purpose

    ADE2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Available Since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCTD26-S>A10-17

    Plasmid

    #112826

    Purpose

    pSMF2 with all S to A mutations in only repeats 10-17.

    Depositor

    Stephen Fuchs

    Article

    Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    pSMF2 with all S to A mutations in only repeats 1…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCTD26-S>A2-9

    Plasmid

    #112825

    Purpose

    pSMF2 with all S to A mutations in only repeats 2-9.

    Depositor

    Stephen Fuchs

    Article

    Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    pSMF2 with all S to A mutations in only repeats 2…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCTD26-S>A18-25

    Plasmid

    #112827

    Purpose

    pSMF2 with all S to A mutations in only repeats 18-25.

    Depositor

    Stephen Fuchs

    Article

    Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    pSMF2 with all S to A mutations in only repeats 1…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS423-SgH

    Plasmid

    #163972

    Purpose

    Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXP812

    Plasmid

    #46062

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJJH1242

    Plasmid

    #36909

    Depositor

    Jürgen Heinisch

    Article

    Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

    Insert

    ScURA3
    

    Use

    Lentiviral

    Expression

    Bacterial, Mammalian, and Y…

    Available Since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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