8,060 results

1. pRS414 Hsh155 WT

   Plasmid

   #87220

   Purpose

   Encodes Hsh155 WT for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGBW-m4046697

   Plasmid

   #153869

   Purpose

   Yeast expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046702

   Plasmid

   #149158

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pGBW-m4046387

   Plasmid

   #149005

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. S3_004_026_MV1

   Plasmid

   #134416

   Purpose

   pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   FdeR-N_2xNLS
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pTDH3

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS316-VTC4

   Plasmid

   #108691

   Purpose

   VTC4 sequence expressed in yeast plasmid

   Depositor

   Michael Downey

   Article

   Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

   Insert

   Vacuolar Transporter Chaperone 4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   VTC4 (native promoter)

   Available Since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4046447

   Plasmid

   #145387

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pKP136 (pRS406-PRC1p-PRC1-PA-ADH1t)

   Plasmid

   #106471

   Purpose

   Expresses Prc1 with a C-terminal PA tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   PRC1
   

   Use

   Tags

   PA

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   PRC1

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCH043

   Plasmid

   #47949

   Purpose

   rtTA under the control of GAL1 promoter

   Depositor

   Attila Becskei

   Article

   Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

   Insert

   rtTA
   

   Use

   Tet inducible expression

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDH5

    Plasmid

    #83775

    Purpose

    Useful for tagging yeast proteins with C-terminal YFP tag; his selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    YFP
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…

    Promoter

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGBW-m4046205

    Plasmid

    #145470

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pMST-1312

    Plasmid

    #118075

    Purpose

    pTPI1-cexA-tCYC1

    Depositor

    Michael Sauer

    Article

    Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

    Insert

    pTPI1-cexA-tCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTPI1

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNC952

    Plasmid

    #20401

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    CFP-reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    X=UbiM (see schematic) linker=deltak

    Promoter

    Available Since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry

    Plasmid

    #68279

    Purpose

    SKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Far1DS NLS mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    RPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG305GAL-ccdB-ECFP

    Plasmid

    #14161

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCH044

    Plasmid

    #47950

    Purpose

    rtTA under the control of GAL2 promoter

    Depositor

    Attila Becskei

    Article

    Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

    Insert

    rtTA
    

    Use

    Tet inducible expression

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal2

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBW-m4046548

    Plasmid

    #149130

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046526

    Plasmid

    #149101

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046386

    Plasmid

    #149089

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046465

    Plasmid

    #149112

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge